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MB Sample ID: SA086219
Local Sample ID: | Sup_Fig4A-6 |
Subject ID: | SU001286 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001286 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Sup_Fig4A-6 | SA086219 | FL012854 | Vitamin D- | Group |
Collection:
Collection ID: | CO001280 |
Collection Summary: | C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University |
Sample Type: | Cecum |
Treatment:
Treatment ID: | TR001301 |
Treatment Summary: | C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University |
Sample Preparation:
Sampleprep ID: | SP001294 |
Sampleprep Summary: | Extract metabolites from cecal contents, then run samples. Pleas see the NMR protocol doc attached to this file |
Sampleprep Protocol Filename: | NMR_protocol.docx |
Analysis:
MB Sample ID: | SA086219 |
Analysis ID: | AN002032 |
Analysis Type: | NMR |
Num Factors: | 4 |
Num Metabolites: | 6 |
Units: | umol/mg |
NMR:
NMR ID: | NM000151 |
Analysis ID: | AN002032 |
Instrument Name: | Bruker Avance II |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 |