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MB Sample ID: SA086576
Local Sample ID: | EL-NYMC-214-Run 4 |
Subject ID: | SU001290 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001290 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
EL-NYMC-214-Run 4 | SA086576 | FL012867 | Early Localized Lyme | Sample Type |
Collection:
Collection ID: | CO001284 |
Collection Summary: | Sera from early Lyme disease patients were collected pretreatment at the initial visit to the clinic. Healthy control serum donors were from endemic and non-endemic regions for Lyme disease. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR001305 |
Treatment Summary: | N/A |
Sample Preparation:
Sampleprep ID: | SP001298 |
Sampleprep Summary: | Small molecule metabolites were extracted from sera and analyzed by LC-MS as previously described. Specifically, cold methanol (60 µl) was added to 20 µl of sera, vortexed and incubated at -20°C for 1 h. Samples were warmed to room temperature for ten minutes, vortexed and centrifuged at 18,000 x g for 30 min. The supernatant (65 µl) was transferred to a new microcentrifuge tube and dried under vacuum. The dried metabolite extract was suspended in 50% methanol (40 µl) centrifuged at 18,000 x g for 15 min. An aliquot (35 µl) was transferred to an autosampler vial for LC-MS analysis. |
Sampleprep Protocol Comments: | doi:10.1038/nprot.2011.335 |
Combined analysis:
Analysis ID | AN002036 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6520 |
Column | Poroshell 120 EC-C8 (100 x 2.1mm,2.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6520 QTOF |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001476 |
Instrument Name: | Agilent 6520 |
Column Name: | Poroshell 120 EC-C8 (100 x 2.1mm,2.5um) |
Column Temperature: | 50 |
Flow Gradient: | 2-98% non-linear gradient |
Flow Rate: | 0.25 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001888 |
Analysis ID: | AN002036 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Agilent MassHunter Qualitative Analysis software ultized for feature detection, Agilent Mass Profiler Professional utilized for feature filtering, and Agilent MassHunter Quantitative analysis software utilized for acquiring feature abundances. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 4000 |
Dry Gas Flow: | 10 l/min |
Dry Gas Temp: | 310 |
Fragment Voltage: | 120 |
Nebulizer: | 45 psi |
Octpole Voltage: | 750 V |
Scanning Range: | 75-1700 |
Skimmer Voltage: | 65 V |