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MB Sample ID: SA090922
Local Sample ID: | SteveD0_010 |
Subject ID: | SU001316 |
Subject Type: | Mammal |
Subject Species: | Equus caballus |
Taxonomy ID: | 9796 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001316 |
Subject Type: | Mammal |
Subject Species: | Equus caballus |
Taxonomy ID: | 9796 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SteveD0_010 | SA090922 | FL013109 | fecal | organ |
SteveD0_010 | SA090922 | FL013109 | D0 | Day |
SteveD0_010 | SA090922 | FL013109 | No | Treatment |
Collection:
Collection ID: | CO001310 |
Collection Summary: | Cecal and fecal samples were obtained before and after (Days days -52, -28, -14, 0, 7, 14, 28 and 52) metronidazole administration. |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR001331 |
Treatment Summary: | Metronidazole (15mg/kg BID PO) was given to horses (n=5) with in-dwelling cecal cannulas. The study was suspended after the fifth dose (day 3) due to severe adverse gastrointestinal effects. |
Sample Preparation:
Sampleprep ID: | SP001324 |
Sampleprep Summary: | Samples were stored at −80 °C until shipped on dry ice to the West Coast Metabolomics Core (University of California, Davis, CA, USA) using an untargeted approach.(20) Samples were homogenized and extracted. Following resuspension in methanol/chloroform, samples were derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons. |
Combined analysis:
Analysis ID | AN002072 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | MALDI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus III GC TOF |
Ion Mode | POSITIVE |
Units | peak intensity |
Chromatography:
Chromatography ID: | CH001509 |
Instrument Name: | Agilent 6890N |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS001923 |
Analysis ID: | AN002072 |
Instrument Name: | Leco Pegasus III GC TOF |
Instrument Type: | GC-TOF |
MS Type: | MALDI |
MS Comments: | Raw data files are preprocessed directly after data acquisition and stored as ChromaTOF-specific *.peg files, as generic *.txt result files and additionally as generic ANDI MS *.cdf files. ChromaTOF vs. 2.32 is used for data preprocessing without smoothing, 3 s peak width, baseline subtraction just above the noise level, and automatic mass spectral deconvolution and peak detection at signal/noise levels of 5:1 throughout the chromatogram. Apex masses are reported for use in the BinBase algorithm. Result *.txt files are exported to a data server with absolute spectra intensities and further processed by a filtering algorithm implemented in the metabolomics BinBase database. |
Ion Mode: | POSITIVE |