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MB Sample ID: SA090944
| Local Sample ID: | SteveD7_012 |
| Subject ID: | SU001316 |
| Subject Type: | Mammal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001316 |
| Subject Type: | Mammal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SteveD7_012 | SA090944 | FL013113 | fecal | organ |
| SteveD7_012 | SA090944 | FL013113 | D7 | Day |
| SteveD7_012 | SA090944 | FL013113 | Yes | Treatment |
Collection:
| Collection ID: | CO001310 |
| Collection Summary: | Cecal and fecal samples were obtained before and after (Days days -52, -28, -14, 0, 7, 14, 28 and 52) metronidazole administration. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001331 |
| Treatment Summary: | Metronidazole (15mg/kg BID PO) was given to horses (n=5) with in-dwelling cecal cannulas. The study was suspended after the fifth dose (day 3) due to severe adverse gastrointestinal effects. |
Sample Preparation:
| Sampleprep ID: | SP001324 |
| Sampleprep Summary: | Samples were stored at −80 °C until shipped on dry ice to the West Coast Metabolomics Core (University of California, Davis, CA, USA) using an untargeted approach.(20) Samples were homogenized and extracted. Following resuspension in methanol/chloroform, samples were derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons. |
Combined analysis:
| Analysis ID | AN002072 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | MALDI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | peak intensity |
Chromatography:
| Chromatography ID: | CH001509 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001923 |
| Analysis ID: | AN002072 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | MALDI |
| MS Comments: | Raw data files are preprocessed directly after data acquisition and stored as ChromaTOF-specific *.peg files, as generic *.txt result files and additionally as generic ANDI MS *.cdf files. ChromaTOF vs. 2.32 is used for data preprocessing without smoothing, 3 s peak width, baseline subtraction just above the noise level, and automatic mass spectral deconvolution and peak detection at signal/noise levels of 5:1 throughout the chromatogram. Apex masses are reported for use in the BinBase algorithm. Result *.txt files are exported to a data server with absolute spectra intensities and further processed by a filtering algorithm implemented in the metabolomics BinBase database. |
| Ion Mode: | POSITIVE |
