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MB Sample ID: SA091967
| Local Sample ID: | L. acidophilus_5 |
| Subject ID: | SU001331 |
| Subject Type: | Bacteria |
| Subject Species: | Lactobacillus |
| Taxonomy ID: | 1578 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | American Type Culture Collection (ATCC) |
| Cell Strain Details: | L. acidophilus (ATCC 4357), L. gasseri (ATCC 33323) |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001331 |
| Subject Type: | Bacteria |
| Subject Species: | Lactobacillus |
| Taxonomy ID: | 1578 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | American Type Culture Collection (ATCC) |
| Cell Strain Details: | L. acidophilus (ATCC 4357), L. gasseri (ATCC 33323) |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| L. acidophilus_5 | SA091967 | FL013187 | data1 | Factor |
Collection:
| Collection ID: | CO001325 |
| Collection Summary: | Cultured in MRS media from frozen glycerol stocks for 24 hours. Harvested, washed, and resuspended in PBS-based buffer solution. Individual cultures treated as biological replicates. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -80℃ |
| Collection Tube Temp: | -80C |
Treatment:
| Treatment ID: | TR001346 |
| Treatment Summary: | No treatment. Simply a metabolomic comparison between species. |
Sample Preparation:
| Sampleprep ID: | SP001339 |
| Sampleprep Summary: | Normalized to 75 mg/mL in PBS-based buffer solution. Metabolites extracted by protein precipitation using 8:1:1 acetonitrile:methanol:acetone. Lipids extracted using the Folch method with 4:2:1 chloroform:methanol:water. |
Combined analysis:
| Analysis ID | AN002096 | AN002097 | AN002098 | AN002099 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity | Intensity | Intensity |
Chromatography:
| Chromatography ID: | CH001531 |
| Chromatography Summary: | Gradient elution was performed with 0.1% formic acid in water as solvent A and acetonitrile as solvent B, at a flow rate of 0.35 mL/min, as such: 0 - 3 min: 100% A, 3 - 13 min: 100% → 20% A, 13 - 16.5 min: 20% A, 16.5 - 20 min: 100% A at 0.6 mL/min (column flush & equilibration). |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Column Temperature: | 25 |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001532 |
| Chromatography Summary: | Gradient elution was performed with 60:40 acetonitrile:water with 0.1% formic acid and 10mM ammonium formate as solvent A and 90:8:2 isopropanol:acetonitrile:water with 0.1% formic acid and 10mM ammonium formate as solvent B, at a flow rate of 0.5 mL/min, as such: 0 - 1 min: 80% A, 1 - 3 min: 80% → 70% A, 3 - 4 min: 70% → 55% A, 4 - 6 min: 55% → 40% A, 6 - 8 min: 40% → 35% A, 8 - 10 min: 35% A, 10 -15 min: 35% → 10% A, 15 - 17 min: 10% → 2% A, 17 - 18 min: 2% A, 18 - 19 min: 2% → 80% A, 19 - 23 min: 80% A (column flush & equilibration) |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001947 |
| Analysis ID: | AN002096 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See manuscript |
| Ion Mode: | POSITIVE |
| MS ID: | MS001948 |
| Analysis ID: | AN002097 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See manuscript |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001949 |
| Analysis ID: | AN002098 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See manuscript |
| Ion Mode: | POSITIVE |
| MS ID: | MS001950 |
| Analysis ID: | AN002099 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See manuscript |
| Ion Mode: | NEGATIVE |
