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MB Sample ID: SA092395
Local Sample ID: | 20post |
Subject ID: | SU001339 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Subject:
Subject ID: | SU001339 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20post | SA092395 | FL013218 | post | Treatment |
Collection:
Collection ID: | CO001333 |
Collection Summary: | 40 urine samples were analyzed in 5 batches. There are 3 labels: control group volunteers (8 pcs., CG), colorectal cancer patients before surgical operation (20 pcs., pre) and after surgical operation (12 pcs., post). |
Sample Type: | Urine |
Storage Conditions: | -20℃ |
Treatment:
Treatment ID: | TR001354 |
Treatment Summary: | All morning fasting urine samples (on an empty stomach, immediately after sleep) from the State Scientific Center of Coloproctology (Moscow, Russia) were collected in sterilized plastic sealed falcons for this study and were frozen in the refrigerator at -20 °C. Then the samples in containers with cold cells were transported to the laboratory. In control group patients with fistula, hemorrhoid, diverticulosis, irritable bowel syndrome and anal fissure were included. In the sample group after the operation, samples were collected no more than three days after the operation. In the sample group before the operation, patients with different histological stages (G1-G3) were involved. The age and sex of the collected subjects were matched as much as possible. All the samples were stored at -24 ˚C until analysis and were defrosted at room temperature prior the analysis. |
Sample Preparation:
Sampleprep ID: | SP001347 |
Sampleprep Summary: | Prior to analysis, samples were defrosted at room temperature and centrifuged at 16000 rpm for 15 min, then aliquots of the supernatant were diluted in 5-fold by deionized water with a final papaverine concentration of 0.2 µg mL-1 and centrifuged at 16000 rpm for 15 min and then aliquots of supernatant were placed into vials. |
Combined analysis:
Analysis ID | AN002111 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
MS Type | ESI |
MS instrument type | Other |
MS instrument name | Shimadzu LCMS-IT-TOF |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH001541 |
Chromatography Summary: | Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5.0% solvent B, elution was performed according to the following conditions: from 5.0% B to 59% B in 30 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. The flow rate was 0.3 mL•min-1. The injection volume was 5 μL. |
Chromatography Comments: | Acquity UPLC BEH; 1.7 μm 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column |
Instrument Name: | Shimadzu 20AD |
Column Name: | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
Column Pressure: | 300-500 bar |
Column Temperature: | 30 |
Flow Gradient: | non-linear |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 5 |
Internal Standard: | papaverine |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001962 |
Analysis ID: | AN002111 |
Instrument Name: | Shimadzu LCMS-IT-TOF |
Instrument Type: | Other |
MS Type: | ESI |
MS Comments: | Mass range: 100 – 850 Da (SCAN mode). Other MS parameters were default (manufacturer recommendations). |
Ion Mode: | POSITIVE |
Capillary Voltage: | 1.55 kV |
Dry Gas Flow: | 100 kPa |
Source Temperature: | 200 |
Cdl Temperature: | 200 |
Interface Voltage: | 4.5 kV |