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MB Sample ID: SA092422

Local Sample ID:18pre
Subject ID:SU001339
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001339
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
18preSA092422FL013219preTreatment

Collection:

Collection ID:CO001333
Collection Summary:40 urine samples were analyzed in 5 batches. There are 3 labels: control group volunteers (8 pcs., CG), colorectal cancer patients before surgical operation (20 pcs., pre) and after surgical operation (12 pcs., post).
Sample Type:Urine
Storage Conditions:-20℃

Treatment:

Treatment ID:TR001354
Treatment Summary:All morning fasting urine samples (on an empty stomach, immediately after sleep) from the State Scientific Center of Coloproctology (Moscow, Russia) were collected in sterilized plastic sealed falcons for this study and were frozen in the refrigerator at -20 °C. Then the samples in containers with cold cells were transported to the laboratory. In control group patients with fistula, hemorrhoid, diverticulosis, irritable bowel syndrome and anal fissure were included. In the sample group after the operation, samples were collected no more than three days after the operation. In the sample group before the operation, patients with different histological stages (G1-G3) were involved. The age and sex of the collected subjects were matched as much as possible. All the samples were stored at -24 ˚C until analysis and were defrosted at room temperature prior the analysis.

Sample Preparation:

Sampleprep ID:SP001347
Sampleprep Summary:Prior to analysis, samples were defrosted at room temperature and centrifuged at 16000 rpm for 15 min, then aliquots of the supernatant were diluted in 5-fold by deionized water with a final papaverine concentration of 0.2 µg mL-1 and centrifuged at 16000 rpm for 15 min and then aliquots of supernatant were placed into vials.

Combined analysis:

Analysis ID AN002111
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu 20AD
Column ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm)
MS Type ESI
MS instrument type Other
MS instrument name Shimadzu LCMS-IT-TOF
Ion Mode POSITIVE
Units Area

Chromatography:

Chromatography ID:CH001541
Chromatography Summary:Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5.0% solvent B, elution was performed according to the following conditions: from 5.0% B to 59% B in 30 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. The flow rate was 0.3 mL•min-1. The injection volume was 5 μL.
Chromatography Comments:Acquity UPLC BEH; 1.7 μm 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column
Instrument Name:Shimadzu 20AD
Column Name:ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm)
Column Pressure:300-500 bar
Column Temperature:30
Flow Gradient:non-linear
Flow Rate:0.3 ml/min
Injection Temperature:5
Internal Standard:papaverine
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001962
Analysis ID:AN002111
Instrument Name:Shimadzu LCMS-IT-TOF
Instrument Type:Other
MS Type:ESI
MS Comments:Mass range: 100 – 850 Da (SCAN mode). Other MS parameters were default (manufacturer recommendations).
Ion Mode:POSITIVE
Capillary Voltage:1.55 kV
Dry Gas Flow:100 kPa
Source Temperature:200
Cdl Temperature:200
Interface Voltage:4.5 kV
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