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MB Sample ID: SA094054
| Local Sample ID: | 226727_Ilyonectria_mors-panacis-MS2neg |
| Subject ID: | SU001367 |
| Subject Type: | Fungi |
| Subject Species: | Ilyonectria mors-panacis;Ilyonectria rufa;Ilyonectria robusta;Ilyonectria torresensis;Ilyonectria estremocensis;Neonectria obtusispora |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001367 |
| Subject Type: | Fungi |
| Subject Species: | Ilyonectria mors-panacis;Ilyonectria rufa;Ilyonectria robusta;Ilyonectria torresensis;Ilyonectria estremocensis;Neonectria obtusispora |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 226727_Ilyonectria_mors-panacis-MS2neg | SA094054 | FL013452 | Virulent | Virulence |
Collection:
| Collection ID: | CO001362 |
| Collection Summary: | Fungal isolates obtained from the Canadian Fungal Culture Collection (CFCC) at Agriculture and Agri-Food Canada were grown on potato dextrose agar for 20 days at 21 C. |
| Sample Type: | Fungal cells |
Treatment:
| Treatment ID: | TR001382 |
| Treatment Summary: | All fungal isolates were grown on potato dextrose agar (2.4%, w/v) with 3.47E-5 zinc sulfate heptahydrate and 3.13E-5 copper sulfate. Plates were grown for 20 days at 21 C in the dark. |
Sample Preparation:
| Sampleprep ID: | SP001375 |
| Sampleprep Summary: | Cultures were sampled by taking six 0.5-cm plugs from each plate. Plugs were extracted with 4 mL of ethyl acetate, then dried by centrifugal vacuum evaporation. Dried extracts were reconstituted in 1 mL of 3:1 methanol:water and were filtered through 0.45 um PTFE filters (Chromatographic Specialties, Inc.). Composite quality control samples were made by combining 50 uL from each completed extract. |
Combined analysis:
| Analysis ID | AN002151 | AN002152 | AN002153 | AN002154 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | Integrated peak area | Integrated peak area | Integrated peak area | Integrated peak area |
Chromatography:
| Chromatography ID: | CH001574 |
| Chromatography Summary: | Samples were resolved with a reversed-phase column (C-18) |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0% B for 0.5 minutes, increased to 100% B over 3 minutes, held at 100% B for 2.5 minutes, decreased to 0% B over 0.5 minutes, then held at 0% B for 1 minute |
| Flow Rate: | 0.3 mL/min |
| Sample Injection: | 5 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Capillary Voltage: | 3900 V |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002002 |
| Analysis ID: | AN002151 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired by single-stage LC-HRMS (140,000 resolution) |
| Ion Mode: | POSITIVE |
| MS ID: | MS002003 |
| Analysis ID: | AN002152 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired by untargeted LC-DDA |
| Ion Mode: | POSITIVE |
| MS ID: | MS002004 |
| Analysis ID: | AN002153 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired by single-stage LC-HRMS (140,000 resolution) |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002005 |
| Analysis ID: | AN002154 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were acquired by untargeted LC-DDA |
| Ion Mode: | NEGATIVE |
