Return to study ST001297 main page
MB Sample ID: SA094169
| Local Sample ID: | Low_4 |
| Subject ID: | SU001371 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001371 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Low_4 | SA094169 | FL013461 | 0.3 | Cellulose(%) |
Collection:
| Collection ID: | CO001366 |
| Collection Summary: | Cecum contents were collected and freeze-dried for further analysis. |
| Sample Type: | Cecum |
Treatment:
| Treatment ID: | TR001386 |
| Treatment Summary: | Freeze-dried cecum contents were stored at liquid N2 until metabolomics study. |
Sample Preparation:
| Sampleprep ID: | SP001379 |
| Sampleprep Summary: | Metabolites were extracted from cecum contents using conventional liquid-liquid extraction method. (Folch method) Internal standard were added to the samples before extraction. Nonpolar and polar metabolites were obtained from organic and aqueous phases, respectively. |
Combined analysis:
| Analysis ID | AN002158 | AN002159 | AN002160 | AN002161 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Pursuit C18 (150 x 2.1mm) | Pursuit C18 (150 x 2.1mm) | Zorbax HILIC plus (100 x 2.1mm,3.5um) | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | cps | cps | cps | cps |
Chromatography:
| Chromatography ID: | CH001576 |
| Chromatography Summary: | C18_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Pursuit C18 (150 x 2.1mm) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001577 |
| Chromatography Summary: | C18_neg |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Pursuit C18 (150 x 2.1mm) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001578 |
| Chromatography Summary: | HILIC_pos |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001579 |
| Chromatography Summary: | HILIC_neg |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002007 |
| Analysis ID: | AN002158 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | POSITIVE |
| MS ID: | MS002008 |
| Analysis ID: | AN002159 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002009 |
| Analysis ID: | AN002160 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | POSITIVE |
| MS ID: | MS002010 |
| Analysis ID: | AN002161 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Xcaliber |
| Ion Mode: | NEGATIVE |
