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MB Sample ID: SA094171
Local Sample ID: | Low_2 |
Subject ID: | SU001371 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001371 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Low_2 | SA094171 | FL013461 | 0.3 | Cellulose(%) |
Collection:
Collection ID: | CO001366 |
Collection Summary: | Cecum contents were collected and freeze-dried for further analysis. |
Sample Type: | Cecum |
Treatment:
Treatment ID: | TR001386 |
Treatment Summary: | Freeze-dried cecum contents were stored at liquid N2 until metabolomics study. |
Sample Preparation:
Sampleprep ID: | SP001379 |
Sampleprep Summary: | Metabolites were extracted from cecum contents using conventional liquid-liquid extraction method. (Folch method) Internal standard were added to the samples before extraction. Nonpolar and polar metabolites were obtained from organic and aqueous phases, respectively. |
Combined analysis:
Analysis ID | AN002158 | AN002159 | AN002160 | AN002161 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Pursuit C18 (150 x 2.1mm) | Pursuit C18 (150 x 2.1mm) | Zorbax HILIC plus (100 x 2.1mm,3.5um) | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | cps | cps | cps | cps |
Chromatography:
Chromatography ID: | CH001576 |
Chromatography Summary: | C18_pos |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Pursuit C18 (150 x 2.1mm) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001577 |
Chromatography Summary: | C18_neg |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Pursuit C18 (150 x 2.1mm) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001578 |
Chromatography Summary: | HILIC_pos |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
Chromatography Type: | HILIC |
Chromatography ID: | CH001579 |
Chromatography Summary: | HILIC_neg |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Zorbax HILIC plus (100 x 2.1mm,3.5um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002007 |
Analysis ID: | AN002158 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcaliber |
Ion Mode: | POSITIVE |
MS ID: | MS002008 |
Analysis ID: | AN002159 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcaliber |
Ion Mode: | NEGATIVE |
MS ID: | MS002009 |
Analysis ID: | AN002160 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcaliber |
Ion Mode: | POSITIVE |
MS ID: | MS002010 |
Analysis ID: | AN002161 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Xcaliber |
Ion Mode: | NEGATIVE |