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MB Sample ID: SA094264
Local Sample ID: | HC-HMA-Rag1KO+aIL-22 Ab #15 |
Subject ID: | SU001375 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001375 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
HC-HMA-Rag1KO+aIL-22 Ab #15 | SA094264 | FL013472 | Anti-IL-22 antibody | Factor |
Collection:
Collection ID: | CO001370 |
Collection Summary: | Feces of anti-IL-22 antibody/control antibody-treated HC-HMA mice were harvested. |
Sample Type: | Feces |
Treatment:
Treatment ID: | TR001390 |
Treatment Summary: | GF C57BL/6 mice were colonized with HC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were treated with control or anti-IL-22 antibody twice (day -5 and day -3) before collecting fecal samples. |
Sample Preparation:
Sampleprep ID: | SP001383 |
Sampleprep Summary: | Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
Analysis ID | AN002166 | AN002167 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
MS Type | ESI | ESI |
MS instrument type | Other | Other |
MS instrument name | Agilent G3250AA LC/MSD TOF system | Agilent G3250AA LC/MSD TOF system |
Ion Mode | POSITIVE | NEGATIVE |
Units | nmol/g | nmol/g |
Chromatography:
Chromatography ID: | CH001584 |
Instrument Name: | Agilent 7100 CE |
Column Name: | Fused-silica,50um(cation) |
Internal Standard: | 20 μM of methionine sulfone |
Chromatography Type: | CE |
Chromatography ID: | CH001585 |
Instrument Name: | Agilent 7100 CE |
Column Name: | COSMO(+),50um(anion |
Internal Standard: | 20 μM of D-camphor-10-sulfonic acid (CSA) |
Chromatography Type: | CE |
MS:
MS ID: | MS002015 |
Analysis ID: | AN002166 |
Instrument Name: | Agilent G3250AA LC/MSD TOF system |
Instrument Type: | Other |
MS Type: | ESI |
MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
Ion Mode: | POSITIVE |
MS ID: | MS002016 |
Analysis ID: | AN002167 |
Instrument Name: | Agilent G3250AA LC/MSD TOF system |
Instrument Type: | Other |
MS Type: | ESI |
MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
Ion Mode: | NEGATIVE |