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MB Sample ID: SA094401
| Local Sample ID: | H35-II |
| Subject ID: | SU001380 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 5-28 months |
| Gender: | Male |
| Human Ethnicity: | Hispanic |
| Human Inclusion Criteria: | (1) boys diagnosed with hypospadias with no comorbidities; (2) children undergoing elective circumcision without a clinical diagnosis; (3) age must be between 0 to 28 months; (4) parents' authorization and sign inform consent |
| Human Exclusion Criteria: | (1) being unable to understand spoken and written language in Spanish; (2) not being willing to provide authorization for collection of tissue sample during the surgical repair of hypospadias and circumcision procedure |
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Subject:
| Subject ID: | SU001380 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 5-28 months |
| Gender: | Male |
| Human Ethnicity: | Hispanic |
| Human Inclusion Criteria: | (1) boys diagnosed with hypospadias with no comorbidities; (2) children undergoing elective circumcision without a clinical diagnosis; (3) age must be between 0 to 28 months; (4) parents' authorization and sign inform consent |
| Human Exclusion Criteria: | (1) being unable to understand spoken and written language in Spanish; (2) not being willing to provide authorization for collection of tissue sample during the surgical repair of hypospadias and circumcision procedure |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| H35-II | SA094401 | FL013488 | Type II | Condition |
Collection:
| Collection ID: | CO001375 |
| Collection Summary: | Foreskin samples were collected from children undergoing urethroplasty and boys scheduled for elective circumcision at the University District Hospital, San Juan, Puerto Rico, and HIMA San Pablo Hospital, Caguas, Puerto Rico. Samples were immediately frozen in dry ice and stored at -80 °C. |
| Sample Type: | Foreskin |
Treatment:
| Treatment ID: | TR001395 |
| Treatment Summary: | No treatment. |
Sample Preparation:
| Sampleprep ID: | SP001388 |
| Sampleprep Summary: | Foreskin samples are cut into small pieces with a scalpel (new) on ice. Weight 50 mg of the foreskin for each sample in 1.5 mL Eppendorf tubes. Sample homogenization and extraction of metabolites is perform adding 1 ml of chloroform/MeOH/H2O (2:5:2) to each sample. Samples are sonicated using a polytron for 15 seconds (3X) in a beaker with ice. Shake samples for 15 minutes in a nutating vortex and centrifuge at 13000 rpm for 10 minutes at 4°C. The supernatant is collected and transferred into 1.5 glass vials for drying. For evaporation, use plastic caps with hole caps. Make a little hole in the center for evaporation to take place. Evaporate samples in SpeedVac until samples are completely dried. |
Combined analysis:
| Analysis ID | AN002175 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GCMS-QP2010 ultra |
| Column | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Shimadzu QP2010 Ultra |
| Ion Mode | UNSPECIFIED |
| Units | millimolar |
Chromatography:
| Chromatography ID: | CH001591 |
| Instrument Name: | Shimadzu GCMS-QP2010 ultra |
| Column Name: | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002023 |
| Analysis ID: | AN002175 |
| Instrument Name: | Shimadzu QP2010 Ultra |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Data was processed using GCMS Labsolution data analysis software (Shimadzu) equipped with NIST14/2014/EPA/NIH database. |
| Ion Mode: | UNSPECIFIED |
