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MB Sample ID: SA094653
| Local Sample ID: | 2005-Control-F |
| Subject ID: | SU001384 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6J |
| Age Or Age Range: | Embryonic day 12.5 |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | polypropylene cages |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001384 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6J |
| Age Or Age Range: | Embryonic day 12.5 |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | polypropylene cages |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2005-Control-F | SA094653 | FL013504 | CTRL | Exposure |
| 2005-Control-F | SA094653 | FL013504 | female | Gender |
Collection:
| Collection ID: | CO001379 |
| Collection Summary: | e12.5 placenta samples were collected immediately after dams were euthanized |
| Sample Type: | Placenta |
| Collection Method: | snap freezen in LN2 right after placenta was isolated |
| Collection Location: | Univ. of Missouri Animal Science Research Center |
| Collection Frequency: | 1 time from each animal |
| Volumeoramount Collected: | 50 mg |
| Storage Vials: | 1.5 ml vials |
Treatment:
| Treatment ID: | TR001399 |
| Treatment Summary: | 2 weeks before breeding and during gestation, dams were fed BPA, or BPS, or vehicle till tissue collection at 12.5 DPC |
| Treatment Compound: | Bisphenol A, bisphenol S |
| Treatment Route: | oral |
| Treatment Dose: | 200 microg/kg body weight |
| Treatment Vehicle: | 70% ethanol dry out onto Nabisco Nilla wafer |
| Animal Endp Euthanasia: | yes |
Sample Preparation:
| Sampleprep ID: | SP001392 |
| Sampleprep Summary: | Freshly isolated mouse placneta tissue was snap frozen in liqud nitrogen and no further preparation. Samples were storred at -52C in lyophilyzer and -20C after weighing. Extracts were dried under nitrogen flow and stored at -20C |
| Processing Method: | Frozen tissues were lyophilized for 24 hrs before weighing. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | samples were extracted water, containing 25 μg/ml ribitol, vortex for 20 seconds, sonicated for 15 minutes, incubated at 50C for 1h |
| Extract Enrichment: | Sample tubes were sonicated for 15 minutes at 13000g then collected the supernatantant |
| Extract Storage: | -20℃ |
| Sample Resuspension: | Sample was resuspended in 25 μl of pyridine containing 15 mg/ml methoxyamine hydrochloride |
| Sample Derivatization: | Sample was derivatized with 25 μl MSTFA (N-methyl-N-(trimethyl-silyl)trifluoroacetamide) + 1% TMCS (chlorotrimethylsilane) |
| Sample Spiking: | Ribitol was used in the extraction solvent as an internal standard |
Combined analysis:
| Analysis ID | AN002181 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N;Agilent 5973N |
| Column | DB-5MS (J&W Scientific) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | relative instrument response |
Chromatography:
| Chromatography ID: | CH001596 |
| Chromatography Comments: | Agilent 6890N coupled to an Agilent 5973N |
| Instrument Name: | Agilent 6890N;Agilent 5973N |
| Column Name: | DB-5MS (J&W Scientific) |
| Column Temperature: | 80 C for 2 min, then ramped at 5 C /min to 315 C and held at 315 C for 12 min |
| Flow Rate: | 1mL/Min of Helium gas |
| Injection Temperature: | 280 C |
| Internal Standard: | Ribitol |
| Sample Injection: | split injection (split ration 5:1) with 1uL injection volume |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002028 |
| Analysis ID: | AN002181 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | 70 eV electron ionization |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 230 C |
| Mass Accuracy: | 0.1 m/z |
| Dataformat: | .D |
| Scan Range Moverz: | 50 to 650 m/z |
