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MB Sample ID: SA096336
| Local Sample ID: | C5 |
| Subject ID: | SU001401 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 2 Months |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001401 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 2 Months |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C5 | SA096336 | FL013588 | No Exposure | Exposure |
| C5 | SA096336 | FL013588 | Control | Days Post Exposure |
| C5 | SA096336 | FL013588 | F | Sex |
Collection:
| Collection ID: | CO001396 |
| Collection Summary: | 2 month old mice were exposed to sonication-induced trauma. Optic nerves were dissected from optic nerve head to optic chiasm. |
| Sample Type: | Neurons |
Treatment:
| Treatment ID: | TR001416 |
| Treatment Summary: | 4 groups of 8 mice, 4 males and 4 females per group. Control group was euthanized at 2 months. Other three groups were exposed to sonication to induce traumatic optic neuropathy. Then groups were euthanized at one day, seven days, and fourteen days, respectively. |
Sample Preparation:
| Sampleprep ID: | SP001409 |
| Sampleprep Summary: | Samples were homogenized and mitochondria were isolated. Lipids were extracted using butanol/methanol lipid extraction and run through mass spectrometer. |
Combined analysis:
| Analysis ID | AN002209 | AN002210 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 600 | Thermo Accela 600 |
| Column | Thermo Acclaim C18 (150 x 2.1mm,3m) | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Main area | Main area |
Chromatography:
| Chromatography ID: | CH001620 |
| Instrument Name: | Thermo Accela 600 |
| Column Name: | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002055 |
| Analysis ID: | AN002209 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002056 |
| Analysis ID: | AN002210 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
| Ion Mode: | POSITIVE |
