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MB Sample ID: SA096347
Local Sample ID: | D14_5 |
Subject ID: | SU001401 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 2 Months |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001401 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 2 Months |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
D14_5 | SA096347 | FL013591 | Sonication | Exposure |
D14_5 | SA096347 | FL013591 | Day Fourteen | Days Post Exposure |
D14_5 | SA096347 | FL013591 | M | Sex |
Collection:
Collection ID: | CO001396 |
Collection Summary: | 2 month old mice were exposed to sonication-induced trauma. Optic nerves were dissected from optic nerve head to optic chiasm. |
Sample Type: | Neurons |
Treatment:
Treatment ID: | TR001416 |
Treatment Summary: | 4 groups of 8 mice, 4 males and 4 females per group. Control group was euthanized at 2 months. Other three groups were exposed to sonication to induce traumatic optic neuropathy. Then groups were euthanized at one day, seven days, and fourteen days, respectively. |
Sample Preparation:
Sampleprep ID: | SP001409 |
Sampleprep Summary: | Samples were homogenized and mitochondria were isolated. Lipids were extracted using butanol/methanol lipid extraction and run through mass spectrometer. |
Combined analysis:
Analysis ID | AN002209 | AN002210 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Accela 600 | Thermo Accela 600 |
Column | Thermo Acclaim C18 (150 x 2.1mm,3m) | Thermo Acclaim C18 (150 x 2.1mm,3m) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE | POSITIVE |
Units | Main area | Main area |
Chromatography:
Chromatography ID: | CH001620 |
Instrument Name: | Thermo Accela 600 |
Column Name: | Thermo Acclaim C18 (150 x 2.1mm,3m) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002055 |
Analysis ID: | AN002209 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
Ion Mode: | NEGATIVE |
MS ID: | MS002056 |
Analysis ID: | AN002210 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
Ion Mode: | POSITIVE |