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MB Sample ID: SA097935
Local Sample ID: | cow 21_Day 21 |
Subject ID: | SU001423 |
Subject Type: | Mammal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001423 |
Subject Type: | Mammal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Gender: | Female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
cow 21_Day 21 | SA097935 | FL013738 | 21 | Day |
cow 21_Day 21 | SA097935 | FL013738 | control | Group |
cow 21_Day 21 | SA097935 | FL013738 | primiparous | Parity |
Collection:
Collection ID: | CO001418 |
Collection Summary: | Blood samples were collected before the morning feeding from the jugular vein of all animals using vacutainer tubes. Samples were allowed to clot at 25°C for 2 h and all tubes were then centrifuged at 2000 × g at 4°C for 15 min to separate serum. Serum was pipetted into 2 mL tubes and stored at -80°C for further analysis. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR001438 |
Treatment Summary: | The feed contained either no additive as a control (CON) or a mineral-based endotoxin binder (EB). |
Sample Preparation:
Sampleprep ID: | SP001431 |
Sampleprep Summary: | Determination of the serum metabolome was carried out using a targeted metabolomics approach based on the Biocrates MxP® Quant 500 kit. Ten μL aliquots of the serum samples were processed according to the manufacturer’s instructions. |
Combined analysis:
Analysis ID | AN002243 | AN002244 | AN002245 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | None (Direct infusion) |
Chromatography system | Agilent 1290 | Agilent 1290 | Agilent 1290 |
Column | - | - | - |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 6500+ QTrap | ABI Sciex 6500+ QTrap | ABI Sciex 6500+ QTrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | µM | µM | µM |
Chromatography:
Chromatography ID: | CH001649 |
Chromatography Summary: | RP-LC-MS/MS |
Instrument Name: | Agilent 1290 |
Column Name: | - |
Column Temperature: | 50 °C |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile; 0.2% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001650 |
Chromatography Summary: | FIA-MS/MS |
Instrument Name: | Agilent 1290 |
Column Name: | - |
Column Temperature: | 25 °C |
Solvent A: | none |
Solvent B: | 290 mL of methanol plus 10 mL of Biocrates' FIA solvent ampule |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002089 |
Analysis ID: | AN002243 |
Instrument Name: | ABI Sciex 6500+ QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
MS ID: | MS002090 |
Analysis ID: | AN002244 |
Instrument Name: | ABI Sciex 6500+ QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |
MS ID: | MS002091 |
Analysis ID: | AN002245 |
Instrument Name: | ABI Sciex 6500+ QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |