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MB Sample ID: SA099111
Local Sample ID: | 15_9 |
Subject ID: | SU001438 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001438 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
15_9 | SA099111 | FL014049 | Donor | Condition |
15_9 | SA099111 | FL014049 | M | Gender |
Collection:
Collection ID: | CO001433 |
Collection Summary: | Donor hearts were procured but not used for heart transplantation (reasons included transportation logistics, donor-recipient mismatch in size, and immune incompatibility), whilst heart failure samples comprised those patients undergoing heart transplantation. |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR001453 |
Treatment Summary: | LV samples from both donors and heart failure patients were collected and snap frozen (-196 °C) immediately within the operating theatre. All samples were stored in the Sydney Heart Bank at -170 to -180°C from the time of harvest. |
Sample Preparation:
Sampleprep ID: | SP001446 |
Sampleprep Summary: | These samples are not post-mortem. Frozen heart tissues were crushed to powder in liquid nitrogen and weighed for metabolomic (~150 mg) analysis. |
Combined analysis:
Analysis ID | AN002270 | AN002271 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1260 | Agilent 1260 |
Column | Waters Atlantis HILIC (100 x 2.1mm,3um,100Å) | Waters XBridge Amide (100 x 4.6mm,3.5um) |
MS Type | API | API |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | intensity (arbitrary unit) | intensity (arbitrary unit) |
Chromatography:
Chromatography ID: | CH001669 |
Instrument Name: | Agilent 1260 |
Column Name: | Waters Atlantis HILIC (100 x 2.1mm,3um,100Å) |
Chromatography Type: | HILIC |
Chromatography ID: | CH001670 |
Instrument Name: | Agilent 1260 |
Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002114 |
Analysis ID: | AN002270 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | API |
MS Comments: | Interface: Turboionspray, positive ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
Ion Mode: | POSITIVE |
MS ID: | MS002115 |
Analysis ID: | AN002271 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | API |
MS Comments: | Interface: Turboionspray, negative ionization mode Scan Mode: Scheduled multiple reaction monitoring (MRM) sMRM Window: 30 sec Target Scan Time: 1.0 sec Source Temp: 450°C Ion Source position: Vertical: 1, Horizontal: 5 |
Ion Mode: | NEGATIVE |