Return to study ST001376 main page
MB Sample ID: SA100520
Local Sample ID: | 28_1uM_Coa_added |
Subject ID: | SU001450 |
Subject Type: | Mammalian Subjects |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | postnatal day 56 |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001450 |
Subject Type: | Mammalian Subjects |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | postnatal day 56 |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
28_1uM_Coa_added | SA100520 | FL014161 | Afternoon | Collection Time |
28_1uM_Coa_added | SA100520 | FL014161 | 0.03 | TCDD treatment(ug/kg) |
Collection:
Collection ID: | CO001445 |
Collection Summary: | Mice were take down with CO2. Livers were excised and immediatley frozen in liquid nitrogen. Collection time (Morning or Afternoon) denoted in sample factors refers to take-down time. Morning cohort samples were collected from when the light turned on (7 am, ZT0) to ZT3.5. Afternoon cohort samples were collected from ZT5-8.5. |
Collection Protocol Comments: | Time-point: 28; Time-point Unit: Days; |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001465 |
Treatment Summary: | oral gavage every 4 days for 28 days. 7 total exposures oral gavage every 4 days for 28 days. 7 total exposures with vehicle or 0.01-30 ug/kg TCDD. |
Treatment Protocol Comments: | Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119 |
Treatment Compound: | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) |
Treatment Route: | GAVAGE |
Treatment Dose: | 0.01,0.03,0.1,0.3,1,3,10,30 |
Treatment Dosevolume: | 0.1 ml |
Treatment Doseduration: | 28 DAYS; |
Treatment Vehicle: | Sesame oil |
Sample Preparation:
Sampleprep ID: | SP001458 |
Sampleprep Summary: | Frozen liver tissue was homegenized in 1000 ul h20:methanol (5:3) mixture5 ml and homegenized, 625 ul chloroform was added,vortexed and spun down at 15,000G. 800 ul of top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
Sampleprep Protocol Filename: | SP_190815_Acetyl-CoA_modified_Metabolomics_polarextraction.docx |
Processing Method: | Liquid extraction |
Processing Storage Conditions: | -80 C |
Combined analysis:
Analysis ID | AN002296 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Acquity H (Waters) |
Column | Ascentis Express C18 2.1x5cm (Waters) |
MS Type | ESI |
MS instrument type | TOF |
MS instrument name | Waters Xevo G2 XS QTof |
Ion Mode | NEGATIVE |
Units | nmol/mg total protein |
Chromatography:
Chromatography ID: | CH001686 |
Methods Filename: | CH_190829_prj155_28RDDR_LC-MS_expiriment_settings_Xevo_G2-XS_QTof_NEG-mode.txt |
Instrument Name: | Acquity H (Waters) |
Column Name: | Ascentis Express C18 2.1x5cm (Waters) |
Internal Standard: | Acetyl-1,2-13C2 Coenzyme A Sodium Salt (A172214, Toronto Research Chemicals, Toronto, Canada) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002139 |
Analysis ID: | AN002296 |
Instrument Name: | Waters Xevo G2 XS QTof |
Instrument Type: | TOF |
MS Type: | ESI |
MS Comments: | Samples were run in MS^e mode. MassLynx V4.2 was used to measure area under the curve. Accurate mass and retention were used for identification of signal for respective metabolites. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_170814_prj140_28RDDR_liver_compound_measurements_QTOF1_NEG.txt |