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MB Sample ID: SA100654
| Local Sample ID: | 64_1uM_Coa_added |
| Subject ID: | SU001450 |
| Subject Type: | Mammalian Subjects |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001450 |
| Subject Type: | Mammalian Subjects |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 64_1uM_Coa_added | SA100654 | FL014175 | Morning | Collection Time |
| 64_1uM_Coa_added | SA100654 | FL014175 | 3 | TCDD treatment(ug/kg) |
Collection:
| Collection ID: | CO001445 |
| Collection Summary: | Mice were take down with CO2. Livers were excised and immediatley frozen in liquid nitrogen. Collection time (Morning or Afternoon) denoted in sample factors refers to take-down time. Morning cohort samples were collected from when the light turned on (7 am, ZT0) to ZT3.5. Afternoon cohort samples were collected from ZT5-8.5. |
| Collection Protocol Comments: | Time-point: 28; Time-point Unit: Days; |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001465 |
| Treatment Summary: | oral gavage every 4 days for 28 days. 7 total exposures oral gavage every 4 days for 28 days. 7 total exposures with vehicle or 0.01-30 ug/kg TCDD. |
| Treatment Protocol Comments: | Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119 |
| Treatment Compound: | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) |
| Treatment Route: | GAVAGE |
| Treatment Dose: | 0.01,0.03,0.1,0.3,1,3,10,30 |
| Treatment Dosevolume: | 0.1 ml |
| Treatment Doseduration: | 28 DAYS; |
| Treatment Vehicle: | Sesame oil |
Sample Preparation:
| Sampleprep ID: | SP001458 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 1000 ul h20:methanol (5:3) mixture5 ml and homegenized, 625 ul chloroform was added,vortexed and spun down at 15,000G. 800 ul of top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Sampleprep Protocol Filename: | SP_190815_Acetyl-CoA_modified_Metabolomics_polarextraction.docx |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80 C |
Combined analysis:
| Analysis ID | AN002296 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Acquity H (Waters) |
| Column | Ascentis Express C18 2.1x5cm (Waters) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Waters Xevo G2 XS QTof |
| Ion Mode | NEGATIVE |
| Units | nmol/mg total protein |
Chromatography:
| Chromatography ID: | CH001686 |
| Methods Filename: | CH_190829_prj155_28RDDR_LC-MS_expiriment_settings_Xevo_G2-XS_QTof_NEG-mode.txt |
| Instrument Name: | Acquity H (Waters) |
| Column Name: | Ascentis Express C18 2.1x5cm (Waters) |
| Internal Standard: | Acetyl-1,2-13C2 Coenzyme A Sodium Salt (A172214, Toronto Research Chemicals, Toronto, Canada) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002139 |
| Analysis ID: | AN002296 |
| Instrument Name: | Waters Xevo G2 XS QTof |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | Samples were run in MS^e mode. MassLynx V4.2 was used to measure area under the curve. Accurate mass and retention were used for identification of signal for respective metabolites. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS_170814_prj140_28RDDR_liver_compound_measurements_QTOF1_NEG.txt |
