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MB Sample ID: SA100890
| Local Sample ID: | mouse_2 |
| Subject ID: | SU001453 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
| Animal Housing: | Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips; |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 22/5 Rodent Diet |
| Animal Water: | Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA |
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Subject:
| Subject ID: | SU001453 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
| Animal Housing: | Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips; |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 22/5 Rodent Diet |
| Animal Water: | Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| mouse_2 | SA100890 | FL014193 | vehicle | TCDD treatment(ug/kg) |
Collection:
| Collection ID: | CO001448 |
| Collection Summary: | Mice were taken down with CO2. Livers were excised and immediately frozen in liquid nitrogen. |
| Collection Protocol Comments: | Time-point: 28; Time-point Unit: DAYS; |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001468 |
| Treatment Summary: | oral gavage every 4 days for 28 days. 7 total exposures with sesame oil vehicle or 0.3-30 ug/kg TCDD. |
| Treatment Protocol Comments: | Administration Interval: 4; Administration Interval Unit: DAYS; Dose Unit: ug/kg; Number of Administrations: 7; Chemical Soucre: AccuStandard; Chemical Catalog Number: S-70982; Chemical Purity: -; Vehicle Substance: CHEBI 28119 |
| Treatment Compound: | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) |
| Treatment Route: | GAVAGE |
| Treatment Dose: | 0.3,1,3,10,30 |
| Treatment Dosevolume: | 0.1 ml |
| Treatment Vehicle: | Sesame oil |
| Animal Fasting: | 2 hrs before end-point |
| Animal Endp Euthanasia: | CO2 |
Sample Preparation:
| Sampleprep ID: | SP001461 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80℃ |
Combined analysis:
| Analysis ID | AN002299 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Ascentis Express C18 (2.1 x 5 cm) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Waters Xevo G2 XS QTof |
| Ion Mode | NEGATIVE |
| Units | normalized signal area |
Chromatography:
| Chromatography ID: | CH001689 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Ascentis Express C18 (2.1 x 5 cm) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002142 |
| Analysis ID: | AN002299 |
| Instrument Name: | Waters Xevo G2 XS QTof |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | Samples were run in MS^e mode. MassLynx V4.2 was used to measure area under the curve. Accurate mass and retention were used for identification of signal for respective metabolites. |
| Ion Mode: | NEGATIVE |
