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MB Sample ID: SA113543
Local Sample ID: | L_K10D28_neg |
Subject ID: | SU001471 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001471 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
L_K10D28_neg | SA113543 | FL014310 | 10 | Litter |
L_K10D28_neg | SA113543 | FL014310 | 28 | Day after birth |
Collection:
Collection ID: | CO001466 |
Collection Summary: | Animals were sacrificed and the liver tissue was removed, snap frozen in liquid nitrogen and stored at -80°C until further analysis |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR001486 |
Treatment Summary: | All C57BL6J wildtype mice were bred locally and held under specific pathogen-free conditions at the Institute of Laboratory Animal Science at RWTH Aachen University Hospital. The day of birth was considered day 0, i.e. animals screened at day 1 were approximately 24 h old and verified to have ingested breast milk (abdominal milk spot). Mice were weaned at PND21. |
Sample Preparation:
Sampleprep ID: | SP001479 |
Sampleprep Summary: | Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. The supernatant was used for the targeted analytics. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65. |
Combined analysis:
Analysis ID | AN002336 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE |
Units | µM |
Chromatography:
Chromatography ID: | CH001712 |
Instrument Name: | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002178 |
Analysis ID: | AN002336 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version Boron from Biocrates |
Ion Mode: | NEGATIVE |