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MB Sample ID: SA122017
| Local Sample ID: | Sample_03 |
| Subject ID: | SU001510 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6 |
| Age Or Age Range: | 10-20 weeks |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001510 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6 |
| Age Or Age Range: | 10-20 weeks |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Sample_03 | SA122017 | FL014922 | Western diet + Lactobacillus johnsonii | Treatment |
Collection:
| Collection ID: | CO001505 |
| Collection Summary: | After the end of the experiment, whole blood was collected in BD Microtainer Serum Separator Tube and allowed to clot by leaving undistubred at room temperature for 30 minutes. Then the tubes were centrifuged at 2000 x g for 10 minutes in 4°C and the supernatant was immediately transferred into a polypropylene tube and stored at -80°C until further analysis. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001525 |
| Treatment Summary: | Western diet fed mice were supplemented with Lactobacillus gasseri or Lactobacillus johnsonii. For control groups, equal volume of vehicle (PBS) was gavaged. Supplementation was done for 2 to 8 weeks. |
Sample Preparation:
| Sampleprep ID: | SP001518 |
| Sampleprep Summary: | Metabolites were extracted with 4 volumes of cold methanol/acetonitrile (1:1, v/v). To precipitate proteins, the samples were incubated for 1h at -20°C. After the samples were centrifuged at 4°C for 15 min at 13,000 rpm, the supernatant was collected and evaporated to dryness in a vacuum concentrator. The dry extracts were then reconstituted in 90 μL of acetonitrile/H2O (1:1, v/v) containing 10 ng/mL CUDA (12-[[(cyclohexylamino)carbonyl] amino]-dodecanoic acid). |
Combined analysis:
| Analysis ID | AN002399 | AN002400 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Poroshell EC-C18 (100 x 3.0 mm,2.7um) | Poroshell EC-C18 (100 x 3.0 mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QToF | Agilent 6545 QToF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AUC | AUC |
Chromatography:
| Chromatography ID: | CH001763 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Poroshell EC-C18 (100 x 3.0 mm,2.7um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.4 ml/min |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002240 |
| Analysis ID: | AN002399 |
| Instrument Name: | Agilent 6545 QToF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | None |
| Ion Mode: | POSITIVE |
| MS ID: | MS002241 |
| Analysis ID: | AN002400 |
| Instrument Name: | Agilent 6545 QToF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | None |
| Ion Mode: | NEGATIVE |
