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MB Sample ID: SA124304
Local Sample ID: | 03_A2_Spleen_naive_0days_170427_UKy_GCH_rep3-polar-NMR_A |
Subject ID: | SU001539 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001539 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
03_A2_Spleen_naive_0days_170427_UKy_GCH_rep3-polar-NMR_A | SA124304 | FL015042 | naive | Treatment Protocol |
Collection:
Collection ID: | CO001534 |
Collection Summary: | Mouse is sacrificed and tissues are harvested. |
Collection Protocol ID: | mouse_tissue_collection |
Sample Type: | Multiple tissues |
Treatment:
Treatment ID: | TR001554 |
Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
Sampleprep ID: | SP001547 |
Sampleprep Summary: | Tissue is frozen in liquid nitrogen to stop metabolic processes. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Polar extraction from homogenate, lypholized, and frozen. Protein extraction and quantification. |
Sampleprep Protocol ID: | tissue_quench; polar_extraction; protein_extraction; frozen_tissue_grind |
Sampleprep Protocol Filename: | 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
Analysis:
MB Sample ID: | SA124304 |
Analysis ID: | AN002440 |
Analysis Type: | NMR |
Num Factors: | 3 |
Num Metabolites: | 258 |
Units: | normalized peak area |
NMR:
NMR ID: | NM000179 |
Analysis ID: | AN002440 |
Instrument Name: | Agilent 600 |
Instrument Type: | FT-NMR |
NMR Experiment Type: | PRESAT (1H1D) |
Standard Concentration: | 0.5 mM |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | cryogenic triple resonance HCN |
NMR Solvent: | D20 |
NMR Tube Size: | 1.7 mm |
Shimming Method: | gradient shimming |
Pulse Sequence: | PRESAT |
Water Suppression: | presaturation |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 15 celsius |
Acquisition Time: | 2 s |
Relaxation Delay: | 4 s |
Baseline Correction Method: | Bernstein Polynomial |