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MB Sample ID: SA125339
Local Sample ID: | OB1 4 (41) |
Subject ID: | SU001561 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001561 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
OB1 4 (41) | SA125339 | FL015354 | KO | Genotype |
Collection:
Collection ID: | CO001556 |
Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. The brain was removed and rinsed with saline solution (SABAX PBS; 0.9% NaCl (w/v), #7634, Adcock Ingram) to remove surrounding blood. The brain regions of interest, namely the anterior cortex (AC), brainstem (BS), cerebellum (CB) and olfactory bulbs (OB), were then dissected, snap-frozen in liquid nitrogen (within 15 minutes postmortem) and stored at − 80°C until used. |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR001576 |
Treatment Summary: | The animals did not receive any treatment |
Sample Preparation:
Sampleprep ID: | SP001569 |
Sampleprep Summary: | Brain regions were homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a biphasic Bligh–Dyer extraction method (methanol/water/chloroform, 2:1.8:2). The polar phases of biphasic extracts were used for LC-MS/MS analysis |
Sampleprep Protocol Filename: | GC_sample_prep_protocol.pdf Data_processing_method.pdf |
Combined analysis:
Analysis ID | AN002464 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1200 |
Column | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410 QQQ |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH001806 |
Instrument Name: | Agilent 1200 |
Column Name: | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002284 |
Analysis ID: | AN002464 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | The Agilent© MassHunter Workstation (v B02.01) and MassHunter Optimiser (v B02.01) software were used for data acquisition in the multiple reaction monitoring (MRM) configuration setting with parameters set for target amino acids, -derivatives, acylcarnitines, and isotopes. Data extraction was done using the Agilent© MassHunter Workstation software (v B06.00; Qualitative Analysis and Quantitative Analysis) |
Ion Mode: | POSITIVE |