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MB Sample ID: SA151942
Local Sample ID: | ctl_1 |
Subject ID: | SU001734 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | K-12 W3110 |
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Subject:
Subject ID: | SU001734 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | K-12 W3110 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
ctl_1 | SA151942 | FL017872 | control | Treatment |
Collection:
Collection ID: | CO001727 |
Collection Summary: | E. coli strain K-12 W3110 (ATCC 27325) was cultured on TSA agar medium. A single bacterial colony was picked from TSA agar medium, and then used to inoculate Luria-Bertani broth (EMD Millipore, Billerica, MA). After being incubated at 37 °C for 12 h on a rotary shaker set to 200 rpm, E. coli cells were harvested at an optical density (OD600) of 1, and then centrifuged at 7,500 × g for 10 min in 50 mL centrifuge tubes. After decanting the supernatant, the pellet was washed with phosphate buffered saline (PBS), and then re-suspended to the volume of bacterial culture for further treatment and analysis. |
Collection Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Sample Type: | Bacterial cells |
Treatment:
Treatment ID: | TR001747 |
Treatment Summary: | E.coli K-12 was treated by a customized IPL instrument for 0 - 20 seconds. |
Treatment Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Sample Preparation:
Sampleprep ID: | SP001740 |
Sampleprep Summary: | After IPL treatment, the cells were centrifuged and washed with PBS twice. The pellets then went though methanol-water-chloroform extraction for obtaining the organic/non-polar phase. |
Sampleprep Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Combined analysis:
Analysis ID | AN002705 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Xevo-G2-S |
Ion Mode | POSITIVE |
Units | no applicable unites |
Chromatography:
Chromatography ID: | CH001996 |
Chromatography Summary: | analysis of lipids |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
Flow Rate: | 0.5 mL/min |
Solvent A: | 60% water/40% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 100% methanol; 0.1% formic acid; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002502 |
Analysis ID: | AN002705 |
Instrument Name: | Waters Xevo-G2-S |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Ion features were captured by MakerLynx (Waters) after eliminating noises. |
Ion Mode: | POSITIVE |