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MB Sample ID: SA151944
| Local Sample ID: | ctl_4 |
| Subject ID: | SU001734 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | K-12 W3110 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001734 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | K-12 W3110 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ctl_4 | SA151944 | FL017872 | control | Treatment |
Collection:
| Collection ID: | CO001727 |
| Collection Summary: | E. coli strain K-12 W3110 (ATCC 27325) was cultured on TSA agar medium. A single bacterial colony was picked from TSA agar medium, and then used to inoculate Luria-Bertani broth (EMD Millipore, Billerica, MA). After being incubated at 37 °C for 12 h on a rotary shaker set to 200 rpm, E. coli cells were harvested at an optical density (OD600) of 1, and then centrifuged at 7,500 × g for 10 min in 50 mL centrifuge tubes. After decanting the supernatant, the pellet was washed with phosphate buffered saline (PBS), and then re-suspended to the volume of bacterial culture for further treatment and analysis. |
| Collection Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001747 |
| Treatment Summary: | E.coli K-12 was treated by a customized IPL instrument for 0 - 20 seconds. |
| Treatment Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Sample Preparation:
| Sampleprep ID: | SP001740 |
| Sampleprep Summary: | After IPL treatment, the cells were centrifuged and washed with PBS twice. The pellets then went though methanol-water-chloroform extraction for obtaining the organic/non-polar phase. |
| Sampleprep Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Combined analysis:
| Analysis ID | AN002705 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo-G2-S |
| Ion Mode | POSITIVE |
| Units | no applicable unites |
Chromatography:
| Chromatography ID: | CH001996 |
| Chromatography Summary: | analysis of lipids |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 60% water/40% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B: | 100% methanol; 0.1% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002502 |
| Analysis ID: | AN002705 |
| Instrument Name: | Waters Xevo-G2-S |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Ion features were captured by MakerLynx (Waters) after eliminating noises. |
| Ion Mode: | POSITIVE |
