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MB Sample ID: SA152197
| Local Sample ID: | 10s_3 |
| Subject ID: | SU001741 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Subject Comments: | E. coli strain K-12 W3110 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001741 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Subject Comments: | E. coli strain K-12 W3110 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 10s_3 | SA152197 | FL017896 | IPL 10 s | Treatment |
Collection:
| Collection ID: | CO001734 |
| Collection Summary: | E.coli strain K-12 was cultured in LB broth and harvested at OD=1. After centrifuge, cell pellets were harvested and washed with PBS twice. Then the pellet was re-suspended in PBS for further treatment. |
| Collection Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001754 |
| Treatment Summary: | For each IPL treatment, a 30 mL K-12 suspension in PBS was loaded in a petri dish (15 cm diameter) and then placed under the broad-spectrum light (wavelength 190-1100 nm) in a Z-1000 SteriPulse-XL system (Xenon Corporation, Woburn, MA). The samples were treated with the IPL for 0, 5, 10, 15, and 20 s. |
| Treatment Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Sample Preparation:
| Sampleprep ID: | SP001747 |
| Sampleprep Summary: | The control and IPL-treated E. coli samples were centrifuged for obtaining the pellets, which were then washed with PBS twice. After extraction with methanol-water-chloroform, the polar phase was separated and derivatized with dansyl chloride (DC) for amino acid analysis. |
| Sampleprep Protocol Filename: | Cell_culture_IPL treatment_Sample preparation_method.docx |
Combined analysis:
| Analysis ID | AN002716 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo-G2-S |
| Ion Mode | POSITIVE |
| Units | no applicable unites |
Chromatography:
| Chromatography ID: | CH002004 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002513 |
| Analysis ID: | AN002716 |
| Instrument Name: | Waters Xevo-G2-S |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Features were captured by MarkerLynx (Waters) and processed. The relative intensity of each ion was calculated by normalizing the single ion counts (SIC) versus the total ion counts (TIC) in the whole chromatogram, while setting the TIC arbitrarily to 10,000. |
| Ion Mode: | POSITIVE |
