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MB Sample ID: SA163126
| Local Sample ID: | 200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#52 - 200916_SCD_1_KO_F8_KO_n2 |
| Subject ID: | SU001818 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001818 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#52 - 200916_SCD_1_KO_F8_KO_n2 | SA163126 | FL019426 | Scd1ab-2J | Genotype |
| 200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#52 - 200916_SCD_1_KO_F8_KO_n2 | SA163126 | FL019426 | Fat | Tissue |
Collection:
| Collection ID: | CO001811 |
| Collection Summary: | Mouse tissues were homogenized in PBS pH 7.4 for lipid extraction and UPLC-MS/MS analysis |
| Sample Type: | Tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001831 |
| Treatment Summary: | Tissues from 6-week old male C57BL/6J mice (wildtype or homozygous for the Scd1ab-2J allele) were purchased from Jackson Laboratory. |
Sample Preparation:
| Sampleprep ID: | SP001824 |
| Sampleprep Summary: | Phospholipids were extracted from tissue homogenates by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS. |
| Extract Storage: | -20℃ |
Combined analysis:
| Analysis ID | AN002834 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | ExionLC |
| Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex Qtrap6500+ |
| Ion Mode | NEGATIVE |
| Units | relative intensities |
Chromatography:
| Chromatography ID: | CH002097 |
| Chromatography Summary: | Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an ExionLC UHPLC. |
| Instrument Name: | ExionLC |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002627 |
| Analysis ID: | AN002834 |
| Instrument Name: | ABI Sciex Qtrap6500+ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.7 (Sciex). |
| Ion Mode: | NEGATIVE |
