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MB Sample ID: SA163169
Local Sample ID: | 200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#44 - 200916_SCD_1_KO_F1_WT_n1 |
Subject ID: | SU001818 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001818 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 6 weeks |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#44 - 200916_SCD_1_KO_F1_WT_n1 | SA163169 | FL019430 | WT | Genotype |
200916_SCD_1_KO_in_vivo_PI_dil_1_20_L_dil_1_10_SMF_#44 - 200916_SCD_1_KO_F1_WT_n1 | SA163169 | FL019430 | Fat | Tissue |
Collection:
Collection ID: | CO001811 |
Collection Summary: | Mouse tissues were homogenized in PBS pH 7.4 for lipid extraction and UPLC-MS/MS analysis |
Sample Type: | Tissue |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001831 |
Treatment Summary: | Tissues from 6-week old male C57BL/6J mice (wildtype or homozygous for the Scd1ab-2J allele) were purchased from Jackson Laboratory. |
Sample Preparation:
Sampleprep ID: | SP001824 |
Sampleprep Summary: | Phospholipids were extracted from tissue homogenates by successive addition of PBS pH 7.4, methanol, chloroform, and saline to a final ratio of 14:34:35:17. Evaporation of the organic layer yielded a lipid film that was dissolved in methanol and subjected to UPLC-MS/MS. |
Extract Storage: | -20℃ |
Combined analysis:
Analysis ID | AN002834 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | ExionLC |
Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex Qtrap6500+ |
Ion Mode | NEGATIVE |
Units | relative intensities |
Chromatography:
Chromatography ID: | CH002097 |
Chromatography Summary: | Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an ExionLC UHPLC. |
Instrument Name: | ExionLC |
Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002627 |
Analysis ID: | AN002834 |
Instrument Name: | ABI Sciex Qtrap6500+ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.7 (Sciex). |
Ion Mode: | NEGATIVE |