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MB Sample ID: SA167545
Local Sample ID: | T30-1 |
Subject ID: | SU001879 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001879 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
T30-1 | SA167545 | FL019916 | lung | type |
Collection:
Collection ID: | CO001872 |
Collection Summary: | Whole human lungs were from eleven individuals; 4 were end-stage diseased lungs acquired from the Emory Transplant Center (IRB approval No. 00006248), one was from Cystic Fibrosis Biospecimen Registry at Emory University (IRB approval No. 00095116) and 6 non-diseased post-mortem lungs were obtained through the International Institute for the Advancement of Medicine (IIAM, Edison, NJ) or Novabiosis (Morrisville, NC). |
Sample Type: | Lung |
Treatment:
Treatment ID: | TR001892 |
Treatment Summary: | Tissue materials were processed similarly with a consistent ratio of 1:5 (sample to hexane-ethyl acetate mixture), i.e., 100 mg lung was homogenized in 300 µL water and extracted with 150 µL formic acid and 400 µL hexane-ethyl acetate mixture, while 40 mg thyroid was homogenized in 250 µL water and extracted with 50 µL formic acid and 200 µL hexane-ethyl acetate mixture. Stool samples (100 mg) were homogenized and extracted directly in 50 µL formic acid and 500 µL hexane-ethyl acetate mixture and then processed as plasma samples. The variation of 1:4 from 1:5 in lung extraction was arbitrary in consideration of the lower density of lung as an organ. The internal standards were spiked at final concentration: 1 ng/mL. The sample mixture was shaken vigorously on ice using multi-tube vortexer (VWR VX-2500) for 1 h and centrifuged at 1000 g, 4 °C for 10 min. The sample mixture was chilled during entire extraction procedure. The organic supernatant was transferred to a new tube with 25 mg MgSO4 (≥99.99% pure, Sigma-Aldrich) for testing of QuEChERS based procedure, and vortexed vigorously to remove water. After 10 min centrifugation at 1000 g, 80 µL of the final supernatant was spiked with instrumental internal standards (final concentration: 1 ng/mL) for analysis. |
Sample Preparation:
Sampleprep ID: | SP001885 |
Sampleprep Summary: | Same as treatment |
Combined analysis:
Analysis ID | AN002924 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo Trace 1310 |
Column | Agilent DB5-MS (15m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | raw intensity |
Chromatography:
Chromatography ID: | CH002166 |
Chromatography Summary: | Samples were analyzed with three injections using GC-HRMS with a Thermo Scientific Q Exactive GC hybrid quadrupole Orbitrap mass spectrometer with 2 µL per injection. A capillary DB-5MS column (15 m × 0.25 mm × 0.25 µm film thickness) was used with the following temperature program: hold 75 °C for 1 min, 25 °C/min to 180 °C, 6 °C/min to 250 °C, 20 °C/min to 350 °C and hold for 5 min. The flow rate of the helium carrier gas was 1 mL/min. Ion source and transfer line temperatures were 250°C and 280°C, respectively. Data were collected from 3 to 24.37 min with positive electron ionization (EI) mode (+70 eV), scanning from m/z 85.0000 to 850.0000 with a resolution of 60,000. |
Instrument Name: | Thermo Trace 1310 |
Column Name: | Agilent DB5-MS (15m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS: