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MB Sample ID: SA171104
| Local Sample ID: | BL-LC-MT(s) |
| Subject ID: | SU001916 |
| Subject Type: | Other organism |
| Subject Species: | Gracilaria edulis |
| Taxonomy ID: | 172966 |
| Gender: | Not applicable |
| Species Group: | Rhodophyta |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001916 |
| Subject Type: | Other organism |
| Subject Species: | Gracilaria edulis |
| Taxonomy ID: | 172966 |
| Gender: | Not applicable |
| Species Group: | Rhodophyta |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| BL-LC-MT(s) | SA171104 | FL020609 | Blank(Methanol) | Solvent |
| BL-LC-MT(s) | SA171104 | FL020609 | Sequential | Treatment |
Collection:
| Collection ID: | CO001909 |
| Collection Summary: | Healthy specimens of Gracilaria edulis were collected from Pantai Morib, Selangor, Malaysia. Further, for the correct identification of G. edulis, observation of the characters was carried out according to Guiry & Guiry (2021). |
| Sample Type: | Seaweed |
Treatment:
| Treatment ID: | TR001928 |
| Treatment Summary: | Extracts of Gracilaria edulis were prepared through two different approaches, namely, sequential and direct, following the procedure of Subermaniam et al. (2020). For the sequential process, the solvents were used in the order of increasing polarity viz. ethyl acetate < acetone. For the direct extracts, ethyl acetate and acetone were used separately. |
Sample Preparation:
| Sampleprep ID: | SP001922 |
| Sampleprep Summary: | Essentially, for both approaches, the seaweeds were rinsed sequentially with seawater followed by normal tap and then double distilled water to eradicate dirt and impurities. Clean samples were then dried using a freeze-dryer and later crushed into fine granule powder using an electric grinder. Different fractions of extracts were prepared using 10 grams of each powder to dissolve them in 100 mL of the mentioned solvents. All the prepared mixtures were made homogeneous using a rotating shaker (Yihder LM-530D, Shaker, Taiwan) for 24 hours and finally centrifuged (Eppendorf 5810 R Centrifuge, Germany) at 4000 rpm for 10 min at 4◦C to separate the supernatant. Each of the clear supernatants of the extracts was concentrated via a Rotary evaporator (Thermo Fisher Scientific EYELA N-1200A Rotary Evaporator, Tokyo). A further concentration using a vacuum concentrator (LaboGene, Brigachtal, Germany) was done to obtain a viscous liquid for storage at 4◦C and future experiments. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | 4℃ |
Combined analysis:
| Analysis ID | AN002981 | AN002982 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | GC |
| Chromatography system | Agilent 1290 Infinity | Agilent 7890B |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF |
| Ion Mode | POSITIVE | UNSPECIFIED |
| Units | Area % | m/z |
Chromatography:
| Chromatography ID: | CH002210 |
| Chromatography Summary: | LC-MS |
| Methods Filename: | LCMS_protocol.txt |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Column Pressure: | 45 psi |
| Column Temperature: | 25 °C |
| Flow Rate: | 0.5 mL per minute |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002211 |
| Chromatography Summary: | GC-MS |
| Methods Filename: | GCMS_protocol.txt |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Flow Rate: | 1.5 mL per minute |
| Analytical Time: | 64 minutes |
| Oven Temperature: | 80°C |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002771 |
| Analysis ID: | AN002981 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data processing Comments: NIST Mass Spectral Search Program-2009 version 2. Software/procedures used for feature assignments: Agilent Mass-Hunter Qualitative Analysis B.05.00. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002772 |
| Analysis ID: | AN002982 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data processing Comments: NIST Mass Spectral Search Program-2009 version 2. Software/procedures used for feature assignments: Agilent Mass-Hunter Qualitative Analysis B.05.00. |
| Ion Mode: | UNSPECIFIED |
