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MB Sample ID: SA171656
Local Sample ID: | 840422-04_003 |
Subject ID: | SU001922 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 23-73 |
Gender: | Male and female |
Human Race: | Hispanic and Caucasian |
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Subject:
Subject ID: | SU001922 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 23-73 |
Gender: | Male and female |
Human Race: | Hispanic and Caucasian |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
840422-04_003 | SA171656 | FL020628 | NAFLD-CAU | factor |
Collection:
Collection ID: | CO001915 |
Collection Summary: | Blood was collected as a part of a routine/pre-operation check up, not more than 2 weeks prior to operation day (bariatric surgery) for the NAFLD group and among normal BMI healthy volunteers for the control group. All volunteers were fasted 10-12 hours before collection. Liver obtained during surgery (no preservatives, flash-frozen) |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR001934 |
Treatment Summary: | Subjects were divided into two groups either: Healthy control or Nonalcoholic fatty liver disease (NAFLD) |
Sample Preparation:
Sampleprep ID: | SP001928 |
Sampleprep Summary: | 1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis |
Combined analysis:
Analysis ID | AN002988 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | EI |
MS instrument type | Other |
MS instrument name | ABI Sciex 4000 QTrap |
Ion Mode | UNSPECIFIED |
Units | nM |
Chromatography:
Chromatography ID: | CH002217 |
Chromatography Summary: | Targeted oxylipin analysis |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002778 |
Analysis ID: | AN002988 |
Instrument Name: | ABI Sciex 4000 QTrap |
Instrument Type: | Other |
MS Type: | EI |
MS Comments: | analyzed by a Waters UPLC with a 2.1×150 mm, 1.7 µm Acquity BEH column, ESI (+) and (-) switching and detection by multi-reaction monitoring on an Sciex 6500+ QTRAP mass spectrometer. We validated this method for linearity, limit of detection, limit of quantitation, inter- and intra-day reproducibility, and recovery for all targets, yielding better than 15% relative standard deviation and (spiked) recoveries of greater than 80%. |
Ion Mode: | UNSPECIFIED |