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MB Sample ID: SA171670
| Local Sample ID: | QC_13_NEG |
| Subject ID: | SU001923 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Balb/c |
| Age Or Age Range: | 8-12 weeks old |
| Weight Or Weight Range: | 20-25 g |
| Gender: | Female |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001923 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Balb/c |
| Age Or Age Range: | 8-12 weeks old |
| Weight Or Weight Range: | 20-25 g |
| Gender: | Female |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| QC_13_NEG | SA171670 | FL020630 | QC | class |
Collection:
| Collection ID: | CO001916 |
| Collection Summary: | Anesthesia was induced with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%. Hearts were extracted immediately after cervical dislocation under anesthesia, snap frozen in dry ice and kept at -80°C until analysis. |
| Sample Type: | Cardiac tissue |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR001935 |
| Treatment Summary: | Injections with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ+atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv) |
| Treatment Compound: | Bortezomib (PS-34, Selleck Chemicals, S1013); Carfilzomib (PR-171, Selleck Chemicals, S2853); all-trans-Retinoic-Acid (Sigma-Aldrich, R2625); Captisol (Ligand Pharmaceuticals |
| Treatment Route: | i.v |
| Treatment Dose: | As described in treatment summary |
| Treatment Vehicle: | Captisol |
| Animal Anesthesia: | Induction with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5% |
| Animal Endp Euthanasia: | 1h |
| Animal Endp Tissue Coll List: | 1h |
Sample Preparation:
| Sampleprep ID: | SP001929 |
| Sampleprep Summary: | 1 Take 100 μL of each sample (including QC) into the corresponding 96-well plate; 2 Add 400 μL of precipitant (methanol: acetonitrile = 2:1, V/V) to the sample, seal the membrane with a film sealer, and shake for 1 min; 3 Place the sample in ice water for ultrasonication for 10 min; 4 Place the sonicated sample at -20°C for 2h; 5 Centrifuge the sample in a centrifuge (4,000 rpm * 4°C, 30 min), each sample taken 300 μL to the new 96-well plate; 6 Put the 96-well plate into a freeze dryer and drain it; 7 On the drained plate, add 300 μL of 10% methanol, mix with a Multi-Tube vorterer (parameter setting 2,500HZ, 3 min), and place in ice water for 10 min; 8 Centrifuge for 20 min (4,000 rpm * 4°C), take 250 μL of the supernatant on a new plate; 9 For each sample (excluding preparation QC), take 50 μL of each sample and mix into a QC sample, and the prepared supernatant is ready to be used. |
| Sampleprep Protocol Filename: | MetaboExtract_en.txt |
Combined analysis:
| Analysis ID | AN002989 | AN002990 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Xevo G2 XS QTOF | Waters Xevo G2 XS QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH002218 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002779 |
| Analysis ID: | AN002989 |
| Instrument Name: | Waters Xevo G2 XS QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Capillary and sampling cone voltages were set at 3.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2) |
| Ion Mode: | POSITIVE |
| MS ID: | MS002780 |
| Analysis ID: | AN002990 |
| Instrument Name: | Waters Xevo G2 XS QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Capillary and sampling cone voltages were set at 2.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2) |
| Ion Mode: | NEGATIVE |
