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MB Sample ID: SA171671
Local Sample ID: | QC_09_POS |
Subject ID: | SU001923 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Balb/c |
Age Or Age Range: | 8-12 weeks old |
Weight Or Weight Range: | 20-25 g |
Gender: | Female |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001923 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Balb/c |
Age Or Age Range: | 8-12 weeks old |
Weight Or Weight Range: | 20-25 g |
Gender: | Female |
Animal Feed: | ad libitum |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
QC_09_POS | SA171671 | FL020630 | QC | class |
Collection:
Collection ID: | CO001916 |
Collection Summary: | Anesthesia was induced with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5%. Hearts were extracted immediately after cervical dislocation under anesthesia, snap frozen in dry ice and kept at -80°C until analysis. |
Sample Type: | Cardiac tissue |
Storage Conditions: | Described in summary |
Treatment:
Treatment ID: | TR001935 |
Treatment Summary: | Injections with CFZ (16 mg/kg, iv), BTZ (1 mg/kg, iv), CFZ+atRA (CFZ: 16 mg/kg iv, atRA: 1 mg/kg iv) or Captisol (Vehicle, iv) |
Treatment Compound: | Bortezomib (PS-34, Selleck Chemicals, S1013); Carfilzomib (PR-171, Selleck Chemicals, S2853); all-trans-Retinoic-Acid (Sigma-Aldrich, R2625); Captisol (Ligand Pharmaceuticals |
Treatment Route: | i.v |
Treatment Dose: | As described in treatment summary |
Treatment Vehicle: | Captisol |
Animal Anesthesia: | Induction with Ketamine 80 mg/kg intramuscular and maintained with Isoflurane 0.5% |
Animal Endp Euthanasia: | 1h |
Animal Endp Tissue Coll List: | 1h |
Sample Preparation:
Sampleprep ID: | SP001929 |
Sampleprep Summary: | 1 Take 100 μL of each sample (including QC) into the corresponding 96-well plate; 2 Add 400 μL of precipitant (methanol: acetonitrile = 2:1, V/V) to the sample, seal the membrane with a film sealer, and shake for 1 min; 3 Place the sample in ice water for ultrasonication for 10 min; 4 Place the sonicated sample at -20°C for 2h; 5 Centrifuge the sample in a centrifuge (4,000 rpm * 4°C, 30 min), each sample taken 300 μL to the new 96-well plate; 6 Put the 96-well plate into a freeze dryer and drain it; 7 On the drained plate, add 300 μL of 10% methanol, mix with a Multi-Tube vorterer (parameter setting 2,500HZ, 3 min), and place in ice water for 10 min; 8 Centrifuge for 20 min (4,000 rpm * 4°C), take 250 μL of the supernatant on a new plate; 9 For each sample (excluding preparation QC), take 50 μL of each sample and mix into a QC sample, and the prepared supernatant is ready to be used. |
Sampleprep Protocol Filename: | MetaboExtract_en.txt |
Combined analysis:
Analysis ID | AN002989 | AN002990 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Xevo G2 XS QTOF | Waters Xevo G2 XS QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area |
Chromatography:
Chromatography ID: | CH002218 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002779 |
Analysis ID: | AN002989 |
Instrument Name: | Waters Xevo G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Capillary and sampling cone voltages were set at 3.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2) |
Ion Mode: | POSITIVE |
MS ID: | MS002780 |
Analysis ID: | AN002990 |
Instrument Name: | Waters Xevo G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Capillary and sampling cone voltages were set at 2.0kV and 40.0V respectively. The mass spectrometry data were acquired in Centroid MSE mode. The TOF mass range was from 50-1200Da and the scan time was 0.2s. Peak Extraction and identification was performed with Progenesis QI (version2.2) |
Ion Mode: | NEGATIVE |