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MB Sample ID: SA174639
| Local Sample ID: | 1356_L |
| Subject ID: | SU001945 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Animal Inclusion Criteria: | Pregnant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001945 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Animal Inclusion Criteria: | Pregnant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 1356_L | SA174639 | FL021449 | Control | Cortisol treatment |
| 1356_L | SA174639 | FL021449 | DCA-treated (over 24h) | DCA treatment |
Collection:
| Collection ID: | CO001938 |
| Collection Summary: | Heart tissue was collected from the right ventricle, left ventricle, and intraventricular septum and immediately frozen in liquid nitrogen. |
| Sample Type: | Heart |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001957 |
| Treatment Summary: | Animals in the CORT and CORT+DCA groups were implanted with pellets of cortisol hemisuccinate (Innovative Research, Sarasota, FL) or infused with cortisol hemisuccinate (Solu-Cortef, Pfizer, Inc) using an ambulatory pump (3D Micro Infusion Pump; Strategic Applications Inc.; Lake Villa, IL) to provide dosing of cortisol at 1mg/kg/day beginning on day 115-116 of gestation until the end of the experiment when the animals were sacrificed. This dose of cortisol increased maternal cortisol concentrations to approximately 1.5-fold, mimicking the rise in cortisol in moderate stress. To produce a regular pattern of labor, ewes were infused with oxytocin (Aspen Veterinary Resources) beginning at day 138-140 of gestation; oxytocin was infused over 5 minutes every 30 minutes (820 µU/kg/min iv;) using an infusion pump controlled by a timed controller ChronTrol Corp, Inc). DCA infusion was started 48 hours after the start of oxytocin. DCA was administered as an intravenous bolus of 25 mg/kg over 3 minutes, followed by an infusion of 12.5 mg DCA/kg/hour for 8 hours, and an infusion of 6.25 mg DCA/kg/h for the next 8 hours. These doses are similar to those that significantly decrease circulating lactate concentrations in humans. The timed infusions of oxytocin continued for 72 hours, and the study was terminated after 24 hours of DCA and/or 72 hours of oxytocin infusion, unless ewes were in the process of delivery or birth occurred. |
Sample Preparation:
| Sampleprep ID: | SP001951 |
| Sampleprep Summary: | Heart tissues were cut to ~ 30 mg and weighted. Each tissue was added to 30 µl of D2O with 10/3 mM sodium trimethylsilylpropane-sulfonate (DSS) and transferred to a 4-mm zirconium dioxide rotor with Kel-F cap and PTFE spacer provided by Bruker Biospin. Samples were kept on dry ice during sample preparation. |
| Sampleprep Protocol Filename: | SamplePrepProtocol.pdf |
Analysis:
| MB Sample ID: | SA174639 |
| Analysis ID: | AN003030 |
| Laboratory Name: | Edison Lab |
| Analysis Type: | NMR |
| Software Version: | Topspin 4.0.6 |
| Results File: | Noesy_binned_data.txt UNITS:(relative) |
NMR:
| NMR ID: | NM000209 |
| Analysis ID: | AN003030 |
| Instrument Name: | Bruker NEO 600MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | D2O |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 4mm CMP_HRMAS |
| NMR Solvent: | D2O |
| NMR Tube Size: | 50 ul |
| Shimming Method: | manual |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Receiver Gain: | 101 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 10 |
| Number Of Scans: | 128 |
| Dummy Scans: | 8 |
| Acquisition Time: | 1.70 s |
| Spectral Width: | 16.0240 ppm |
| Num Data Points Acquired: | 32768 |
| Line Broadening: | 1.8 Hz |
| Baseline Correction Method: | ABSG |
| NMR Results File: | Noesy_binned_data.txt UNITS:(relative) |
