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MB Sample ID: SA174982
| Local Sample ID: | Copepodamide 14 |
| Subject ID: | SU001958 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium minutum |
| Taxonomy ID: | 39455 |
| Genotype Strain: | CCMP 113 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001958 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium minutum |
| Taxonomy ID: | 39455 |
| Genotype Strain: | CCMP 113 |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Copepodamide 14 | SA174982 | FL021503 | Copepodamide | Treatment |
Collection:
| Collection ID: | CO001951 |
| Collection Summary: | Alexandrium minutum cells were collected by vacuum filtration onto GF/F filters and quenched with liquid nitrogen. Frozen cells with filters were stored in foil (previously muffled for 3 h at 450 °C) at -80 °C until extraction. |
| Collection Protocol ID: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Sample Type: | Algae |
| Collection Method: | Filtration and Freeze |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001970 |
| Treatment Summary: | Twice, first at the start of the experiment and again 24 h later, 1.5 mL of either 0.2 µM copepodamides (copepod cue) in DMSO (treatment, n=20) or DMSO alone (control, n=20) were added aseptically to 300 mL cultures of Alexandrium minutum resulting in a final concentration of 1 nM copepodamides. |
| Treatment Protocol Filename: | Predator_Cues_Target_Signaling_Pathways_in_Toxic_Algal_Metabolome_protocol.pdf |
Sample Preparation:
| Sampleprep ID: | SP001964 |
| Sampleprep Summary: | Non-polar extracts were dissolved at a concentration equivalent to 2.27 x 10^7 A. minutum cells mL-1 in dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane (TMS), as internal standard. The media blank extract was prepared in the smallest volume of solvent possible (175 µL). The non-polar extracts were transferred to 3 mm NMR tubes, and analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra of non-polar extracts were acquired using a simple 1H NMR experiment (Bruker zg30 pulse sequence) with a spectral width of 9.6 kHz centered at 4400.00 Hz over 320 scans. One treatment replicate was lost and therefore its spectrum was not acquired. |
| Sampleprep Protocol Filename: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
Analysis:
| MB Sample ID: | SA174982 |
| Analysis ID: | AN003041 |
| Analysis Type: | NMR |
| Results File: | Preprocessed_binned_nonpolar_NMRmetabolomics.txt |
| Units: | area under the curve |
NMR:
| NMR ID: | NM000210 |
| Analysis ID: | AN003041 |
| Instrument Name: | Bruker Avance IIIHD |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | 3 mm triple resonance broadband cryoprobe |
| NMR Solvent: | Dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane |
| NMR Tube Size: | 3 mm |
| Pulse Sequence: | zg30 |
| Number Of Scans: | 320 |
| Spectral Width: | 9.6 kHz |
| Baseline Correction Method: | Spline |
| Binned Increment: | 0.005 ppm |
