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MB Sample ID: SA183630
Local Sample ID: | MGH193 |
Subject ID: | SU002025 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Age Or Age Range: | 17 day after germination |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002025 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Age Or Age Range: | 17 day after germination |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MGH193 | SA183630 | FL022452 | Ames 27133 | Pedigree |
Collection:
Collection ID: | CO002018 |
Collection Summary: | Entire shoots of 17-day old maize plants were collected and immediately frozen in liquid nitrogen. All samples were collected within one hour in the middle of the day (12:00-1:00 pm). |
Sample Type: | Plant |
Collection Method: | Cut entire shoots from 1 cm above ground and collected into 50 ml screw cap tube and quickly thrown into liquid N2. |
Collection Location: | University of Nebraska-Lincoln Innovation Campus greenhouse |
Collection Frequency: | once |
Collection Duration: | 8/16/2018 12:00 pm - 1:00 pm |
Volumeoramount Collected: | Entire shoot of a single plant |
Storage Conditions: | -80℃ |
Collection Vials: | 50 ml falcon tube |
Storage Vials: | 50 ml falcon tube |
Collection Tube Temp: | Liquid N2 |
Treatment:
Treatment ID: | TR002037 |
Treatment Summary: | Seeds were surface-sterilized, imbibed, and sown on Fafard germination soil. Plants were and cultured in a greenhouse with a temperature of 18-25 °C and a 14/10 h light/dark cycle for 14 days. All inbred lines were treated equally. |
Plant Growth Support: | Fafard germination soil |
Plant Growth Location: | University of Nebraska-Lincoln Innovation campus greenhouse |
Plant Plot Design: | Randomized |
Plant Light Period: | 14/10 h light/dark cycle |
Plant Temp: | 18-25 °C |
Plant Watering Regime: | daily |
Plant Nutritional Regime: | no nutrient addition during the experimental period |
Plant Estab Date: | 2018-07-31 |
Plant Harvest Date: | 2018-08-13 |
Plant Growth Stage: | Juvenile |
Plant Metab Quench Method: | Quick freezing by liquid nitrogen |
Plant Harvest Method: | cutting |
Sample Preparation:
Sampleprep ID: | SP002031 |
Sampleprep Summary: | Whole samples were ground into fine powder under liquid nitrogen temperature and aliquoted 25 mg for metabolite analysis. The metabolites were extracted by methanol: water: chloroform and 50 µl of the upper aqueous phase was dried down to be derivatized by methoxyamination and trimethylsilylation. |
Processing Storage Conditions: | Room temperature |
Extract Storage: | Room temperature |
Sample Resuspension: | pyridine |
Sample Derivatization: | methoxyamination and trimethylsilylation |
Sample Spiking: | 8.2 ug/ml ribitol |
Combined analysis:
Analysis ID | AN003169 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7200 Q-TOF |
Ion Mode | POSITIVE |
Units | arbitrary unit (relative contents) |
Chromatography:
Chromatography ID: | CH002342 |
Instrument Name: | Agilent 7890B |
Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
Column Temperature: | 80 |
Flow Gradient: | None |
Flow Rate: | 1 ml/min |
Injection Temperature: | 230 |
Time Program: | 80 °C 2 min, ramp up to 330°C at 15 °C/min, hold for 6 min |
Transferline Temperature: | 280℃ |
Sample Syringe Size: | 10 µl |
Randomization Order: | manual |
Chromatography Type: | GC |
MS:
MS ID: | MS002947 |
Analysis ID: | AN003169 |
Instrument Name: | Agilent 7200 Q-TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | MassHunter software suits |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 250 |
Ionization: | EI |
Ionization Energy: | 70 eV |