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MB Sample ID: SA184884
Local Sample ID: | HC_6hr_1 |
Subject ID: | SU002045 |
Subject Type: | Other organism |
Subject Species: | Microchloropsis |
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Subject:
Subject ID: | SU002045 |
Subject Type: | Other organism |
Subject Species: | Microchloropsis |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
HC_6hr_1 | SA184884 | FL022733 | HC | Treatment |
Collection:
Collection ID: | CO002038 |
Collection Summary: | Marine microalgae Microchloropsis gaditana NIES 2587 is procured from Microbial Culture Collection, National Institute for Environmental Studies (NIES), Tsukuba, Japan. The strain was grown in minimal medium F/2 (Guillard and Ryther, 1962) under a light regime of 16:8 h and an illumination of 150 µmol m−2 s−1 photosynthetically active radiation (PAR) in a multi-cultivator MC 1000-OD (Photon Systems Instruments, Czech Republic) with a flow rate of 800 mL min-1 with continuous bubbling of air at 24 °C. |
Sample Type: | Algae |
Treatment:
Treatment ID: | TR002057 |
Treatment Summary: | Microchloropsis gaditana cells were grown in a Multicultivator in the presence of very-low CO2 (300 ppm) and high CO2 (30,000 ppm) for 24 hours with an illumination intensity of 150 uE. |
Treatment Compound: | CO2 |
Sample Preparation:
Sampleprep ID: | SP002051 |
Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |
Processing Storage Conditions: | Described in summary |
Extraction Method: | Sonication |
Sample Derivatization: | MSTFA |
Sample Spiking: | Ribitol (Internal Standard) |
Combined analysis:
Analysis ID | AN003203 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
MS Type | EI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 7890A |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH002368 |
Chromatography Summary: | GC-triple quadrupole analysis was performed on an HP-5 gas chromatograph with standard liners containing glass wool in split mode (1:5) at 250°C injector temperature. The GC was operated at constant flow of 1 ml/min helium on a 30-m, 0.25-mm i.d., 0.25-μm HP-5 column, a start temperature of 60°C, 3 min isothermal, temperature ramping by 5°C/min to 180°C, 3 min isothermal and finally temperature ramping of 10°C/min to 310°C. |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
Chromatography Type: | GC |
MS:
MS ID: | MS002981 |
Analysis ID: | AN003203 |
Instrument Name: | Agilent 7890A |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | Mass Hunter |
Ion Mode: | POSITIVE |