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MB Sample ID: SA188457
Local Sample ID: | QC_GE1_01_3094 |
Subject ID: | SU002095 |
Subject Type: | Bacteria |
Subject Species: | swine colonic microbiota |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002095 |
Subject Type: | Bacteria |
Subject Species: | swine colonic microbiota |
Gender: | Not applicable |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
QC_GE1_01_3094 | SA188457 | FL023194 | QC | group |
QC_GE1_01_3094 | SA188457 | FL023194 | QC | ferm |
Collection:
Collection ID: | CO002088 |
Collection Summary: | The PolyFermS in vitro continuous fermentation model was used here with modified Macfarlane medium to simulate the porcine proximal colon. During 35 days of fermentation, a first-stage reactor containing immobilized swine fecal microbiota fed two second-stage control and test reactors operated in parallel and used to test the effects of MccJ25 on the metabolic activity of the microbiota. |
Sample Type: | Bacterial cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002107 |
Treatment Summary: | Performed simultaneously in TR1 and TR2, the four treatments consisted of adding by adding Salmonella enterica subsp. enterica serovar Newport ATCC 6962 alone at an initial concentration of 107 cfu/mL to each reactor, followed by adding the bacteria at this cell density along with each tested antimicrobial: 0.4 mM MccJ25, 4 mM reuterin or 0.6 mM rifampicin. |
Sample Preparation:
Sampleprep ID: | SP002101 |
Sampleprep Summary: | The metabolites were extracted from swine colonic microbiota in methanol/chloroform/water. The aqueous phase was collected. |
Processing Storage Conditions: | On ice |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN003282 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Thermo Acclaim Polar Advantage II |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker Maxis II ETD |
Ion Mode | POSITIVE |
Units | m/z |
Chromatography:
Chromatography ID: | CH002424 |
Chromatography Summary: | Low pH polar |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Thermo Acclaim Polar Advantage II |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003054 |
Analysis ID: | AN003282 |
Instrument Name: | Bruker Maxis II ETD |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Proprietary analytical software for MS acquisition Data processing with xcms (R package) |
Ion Mode: | POSITIVE |