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MB Sample ID: SA188512
| Local Sample ID: | RifaF1_t4_BB3_01_3114 |
| Subject ID: | SU002095 |
| Subject Type: | Bacteria |
| Subject Species: | swine colonic microbiota |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002095 |
| Subject Type: | Bacteria |
| Subject Species: | swine colonic microbiota |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| RifaF1_t4_BB3_01_3114 | SA188512 | FL023197 | Rifa | group |
| RifaF1_t4_BB3_01_3114 | SA188512 | FL023197 | F1 | ferm |
Collection:
| Collection ID: | CO002088 |
| Collection Summary: | The PolyFermS in vitro continuous fermentation model was used here with modified Macfarlane medium to simulate the porcine proximal colon. During 35 days of fermentation, a first-stage reactor containing immobilized swine fecal microbiota fed two second-stage control and test reactors operated in parallel and used to test the effects of MccJ25 on the metabolic activity of the microbiota. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002107 |
| Treatment Summary: | Performed simultaneously in TR1 and TR2, the four treatments consisted of adding by adding Salmonella enterica subsp. enterica serovar Newport ATCC 6962 alone at an initial concentration of 107 cfu/mL to each reactor, followed by adding the bacteria at this cell density along with each tested antimicrobial: 0.4 mM MccJ25, 4 mM reuterin or 0.6 mM rifampicin. |
Sample Preparation:
| Sampleprep ID: | SP002101 |
| Sampleprep Summary: | The metabolites were extracted from swine colonic microbiota in methanol/chloroform/water. The aqueous phase was collected. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003282 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Thermo Acclaim Polar Advantage II |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Maxis II ETD |
| Ion Mode | POSITIVE |
| Units | m/z |
Chromatography:
| Chromatography ID: | CH002424 |
| Chromatography Summary: | Low pH polar |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Thermo Acclaim Polar Advantage II |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003054 |
| Analysis ID: | AN003282 |
| Instrument Name: | Bruker Maxis II ETD |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Proprietary analytical software for MS acquisition Data processing with xcms (R package) |
| Ion Mode: | POSITIVE |
