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MB Sample ID: SA189386
Local Sample ID: | III.10 |
Subject ID: | SU002103 |
Subject Type: | Plant |
Subject Species: | Alkanna tinctoria |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002103 |
Subject Type: | Plant |
Subject Species: | Alkanna tinctoria |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
III.10 | SA189386 | FL023248 | fruiting | developmental stage |
III.10 | SA189386 | FL023248 | Greek B | soil type |
Collection:
Collection ID: | CO002096 |
Collection Summary: | Alkanna tinctoria plants were provided as rooted acclimatized individuals, originally collected and identified from natural populations |
Sample Type: | Plant |
Treatment:
Treatment ID: | TR002115 |
Treatment Summary: | Plants were produced by micropropagation from several mother plants by the Hellenic Agricultural Organization (HAO, Thessaloniki, Greece). The plants were transferred to 5 L pots containing 4.5 L of sterilized (121° C for 15 min) peat moss and perlite (volume ratio 2:1), mixed with 200 g field soil collected either in Austria or in Greece. Thus, all plants were grown in a substrate with highly similar chemical and physical characteristics, but hosting those microbial communities prevailing in these three distinct soils. Plants were grown in the greenhouse at 16 h light / 8 h dark photoperiod, 25°C with 50% relative humidity (RH) and a photosynthetic photon flux density (PPFD) of 96 μmolm^-2 s^-1. Plants were watered twice per week with deionized water and moved randomly once per week. Plants were harvested at four different defined developmental stages, the first stage (“vegetative growth”) was defined when more than 50% of the individuals started to produce new leaves, “blooming” was the stage when more than 50% of the individuals had flowers, “fruiting” when more than 50% of the individual plants began to produce fruits. |
Sample Preparation:
Sampleprep ID: | SP002109 |
Sampleprep Summary: | Plant roots were ground to a fine powder using a ball mill (Fritsch Pulverisette 0, Germany). Each powdered sample was weighed (70 mg) into microcentrifuge tubes, followed by extraction with 3 mL of methanol by ultrasound at 10% power for 3 h (Bandelin Sonorex Digital 10P, Berlin, Germany) and centrifugation for 10 minutes at 12.500 rpm (Hermle Z 216 MK, Wehingen, Germany). The supernatants were collected and subjected to UHPLC-HRMS analysis after filtering with 0.22 μm syringe filters. |
Combined analysis:
Analysis ID | AN003291 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Accela 1250 |
Column | Waters Acquity UPLC HSS C18 SB (100 x 2.1mm, 1.8um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo LTQ Discovery Orbitrap |
Ion Mode | POSITIVE |
Units | intensity units |
Chromatography:
Chromatography ID: | CH002431 |
Instrument Name: | Thermo Accela 1250 |
Column Name: | Waters Acquity UPLC HSS C18 SB (100 x 2.1mm, 1.8um) |
Column Temperature: | 50 |
Flow Rate: | 0.3mL/min |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003062 |
Analysis ID: | AN003291 |
Instrument Name: | Thermo LTQ Discovery Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The MS/MS data were obtained for the six most intense m/z peaks in each full scan, with the normalized collision energy set to 35 eV. The acquisition and initial processing of the data were by means of XcaliburTM (Thermo Scientific, USA) software, while data alignment and feature extraction were performed utilizing the XCMS Online platform (The Scripps Research Institute, USA). The batch error correction was done with the help of MetaboAnalyst 5.0. |
Ion Mode: | POSITIVE |