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MB Sample ID: SA189430
Local Sample ID: | 236_015 |
Subject ID: | SU002104 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002104 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
236_015 | SA189430 | FL023253 | 2 | Year |
Collection:
Collection ID: | CO002097 |
Collection Summary: | Samples were aliquoted from pooled sera collected from multiple individuals. Samples were purchased from Innovative Research (Novi, MI). |
Sample Type: | Blood (serum) |
Storage Conditions: | -80℃ |
Storage Vials: | Wheaton 2mL Cryovials |
Additives: | Na Heparin |
Treatment:
Treatment ID: | TR002116 |
Treatment Summary: | Samples were not treated, beyond the initial aliquotting of the pooled human sera upon receipt at our laboratory before storage at -80C |
Sample Preparation:
Sampleprep ID: | SP002110 |
Sampleprep Summary: | Prior to spectral acquisition samples were methanol precipitated and ultrafiltered to remove macromolecules prior to spectra acquisition |
Sampleprep Protocol Filename: | Serum_Precipitation_SOP.docx, Serum_Filtration_SOP.docx |
Processing Storage Conditions: | On ice |
Extraction Method: | Methanol Precipitation |
Extract Storage: | -80℃ |
Sample Resuspension: | Samples were resuspended in 50mM phosphate buffer in D2O |
Sample Spiking: | Samples were spiked with a Formate solution in D2O of known concentration |
Analysis:
MB Sample ID: | SA189430 |
Analysis ID: | AN003292 |
Analysis Type: | NMR |
Num Factors: | 3 |
Num Metabolites: | 31 |
Units: | uM |
NMR:
NMR ID: | NM000226 |
Analysis ID: | AN003292 |
Instrument Name: | Varian 500 MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 500 |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Auto shim (gradient shimming), with manual shimming when necessary |
Pulse Sequence: | 1D-NOESY |
Water Suppression: | Saturation at 80Hz induced field strength |
Temperature: | 25 |
Number Of Scans: | 32 |