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MB Sample ID: SA192553
Local Sample ID: | 5003448281 |
Subject ID: | SU002130 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 15-37 |
Gender: | Female |
Human Inclusion Criteria: | pregnant, at the first trimester |
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Subject:
Subject ID: | SU002130 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 15-37 |
Gender: | Female |
Human Inclusion Criteria: | pregnant, at the first trimester |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
5003448281 | SA192553 | FL023667 | ZIKV- | Group |
5003448281 | SA192553 | FL023667 | -1 | Urine timepoint |
Collection:
Collection ID: | CO002123 |
Collection Summary: | At each visit, women were given a physical examination and blood, urine, saliva, and vaginal fluid samples were collected as per ZIP study protocols (see attachment). In between monthly visits, women would also collect a urine sample at home to be brought to the next monthly visit or collected at a biweekly visit to the clinic. |
Collection Protocol Filename: | Lebov_Study_protocol.pdf |
Sample Type: | Urine |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002142 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP002136 |
Sampleprep Summary: | In brief, each sample was centrifuged at 3500g and then 540µl of supernatant was added by 60µl NMR buffer (1.5M KH2PO4 in D2O with a final concentration of 0.33 mM DSS, pH = 7.4). After this, we centrifuged the sample again at 4000g and transferred 590 µl supernatant to the 5mm NMR tubes in racks of 96 tubes (Bruker Biospin, USA). |
Sampleprep Protocol Filename: | zika_01_sz_urine_extraction.pdf |
Analysis:
MB Sample ID: | SA192553 |
Analysis ID: | AN003335 |
Laboratory Name: | Edison Lab |
Analysis Type: | NMR |
Results File: | Zika_binned_mw.txt |
Units: | (relative) |
NMR:
NMR ID: | NM000227 |
Analysis ID: | AN003335 |
Instrument Name: | Bruker 600MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | D2O |
Standard Concentration: | 0.33 mM |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | CryoProbe |
NMR Solvent: | 1.5M KH2PO4 in D2O with a final concentration of 0.33 mM DSS, pH = 7.4 |
NMR Tube Size: | 5 mm |
Shimming Method: | topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | presat |
Pulse Width: | 18.06 |
Power Level: | -7.45 dB |
Receiver Gain: | 90.5 |
Offset Frequency: | 2820.68 |
Presaturation Power Level: | 41.4 dB |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 27 |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 2.72 s |
Relaxation Delay: | 4 |
Spectral Width: | 20.0276 ppm |
Num Data Points Acquired: | 65382 |
Real Data Points: | 65536 |
Line Broadening: | 0.3 |
Chemical Shift Ref Std: | DSS |
Binned Increment: | Manual |