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MB Sample ID: SA192566
| Local Sample ID: | 5003446147 |
| Subject ID: | SU002130 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 15-37 |
| Gender: | Female |
| Human Inclusion Criteria: | pregnant, at the first trimester |
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Subject:
| Subject ID: | SU002130 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 15-37 |
| Gender: | Female |
| Human Inclusion Criteria: | pregnant, at the first trimester |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 5003446147 | SA192566 | FL023668 | ZIKV- | Group |
| 5003446147 | SA192566 | FL023668 | -2 | Urine timepoint |
Collection:
| Collection ID: | CO002123 |
| Collection Summary: | At each visit, women were given a physical examination and blood, urine, saliva, and vaginal fluid samples were collected as per ZIP study protocols (see attachment). In between monthly visits, women would also collect a urine sample at home to be brought to the next monthly visit or collected at a biweekly visit to the clinic. |
| Collection Protocol Filename: | Lebov_Study_protocol.pdf |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002142 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP002136 |
| Sampleprep Summary: | In brief, each sample was centrifuged at 3500g and then 540µl of supernatant was added by 60µl NMR buffer (1.5M KH2PO4 in D2O with a final concentration of 0.33 mM DSS, pH = 7.4). After this, we centrifuged the sample again at 4000g and transferred 590 µl supernatant to the 5mm NMR tubes in racks of 96 tubes (Bruker Biospin, USA). |
| Sampleprep Protocol Filename: | zika_01_sz_urine_extraction.pdf |
Analysis:
| MB Sample ID: | SA192566 |
| Analysis ID: | AN003335 |
| Laboratory Name: | Edison Lab |
| Analysis Type: | NMR |
| Results File: | Zika_binned_mw.txt |
| Units: | (relative) |
NMR:
| NMR ID: | NM000227 |
| Analysis ID: | AN003335 |
| Instrument Name: | Bruker 600MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | D2O |
| Standard Concentration: | 0.33 mM |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | CryoProbe |
| NMR Solvent: | 1.5M KH2PO4 in D2O with a final concentration of 0.33 mM DSS, pH = 7.4 |
| NMR Tube Size: | 5 mm |
| Shimming Method: | topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Pulse Width: | 18.06 |
| Power Level: | -7.45 dB |
| Receiver Gain: | 90.5 |
| Offset Frequency: | 2820.68 |
| Presaturation Power Level: | 41.4 dB |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 27 |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.72 s |
| Relaxation Delay: | 4 |
| Spectral Width: | 20.0276 ppm |
| Num Data Points Acquired: | 65382 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.3 |
| Chemical Shift Ref Std: | DSS |
| Binned Increment: | Manual |
