Return to study ST002057 main page
MB Sample ID: SA193842
Local Sample ID: | NASH_BXW_L1S8 |
Subject ID: | SU002139 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002139 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
NASH_BXW_L1S8 | SA193842 | FL023739 | NASH From Patient | Treatment |
Collection:
Collection ID: | CO002132 |
Collection Summary: | Primary Human Hepatocytes provided by BioIVT. Collected from deceased subjects |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002151 |
Treatment Summary: | NASH hepatocytes were taken from patients with NASH. IVIS Hepatocytes were cultured and treated with fatty acid enriched media |
Treatment Compound: | Free fatty acids |
Cell Growth Config: | 24-well plate |
Sample Preparation:
Sampleprep ID: | SP002145 |
Sampleprep Summary: | Lipidomics extraction using C:W:M extraction followed by ButOH:MeOH of residual culture plate. Extractions were dried down, combined, and reconstituted. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN003349 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Ascentis Express C8,(2.7um 100mm) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | AU |
Chromatography:
Chromatography ID: | CH002480 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Ascentis Express C8,(2.7um 100mm) |
Column Temperature: | 40 |
Flow Rate: | 0.2 mL/min |
Sample Injection: | 10 uL |
Solvent A: | 60% water/40% isopropanol; 2 mM formic acid; 8 mM ammonium carbonate |
Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium carbonate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003118 |
Analysis ID: | AN003349 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The LC-MS raw file conversion was performed using ProteoWizard and XCMS. Mzmatch.R was then used for alignment and filtering of peaks with an intensity cut-off of 100,000, relative standard deviation of less than 0.8, and peak shape noise filter of greater than 0.8. Output was then further processed using IDEOM |
Ion Mode: | UNSPECIFIED |
Ion Spray Voltage: | 3.5 kV |