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MB Sample ID: SA196336
| Local Sample ID: | 21_exudate-9d_1 |
| Subject ID: | SU002160 |
| Subject Type: | Other organism |
| Subject Species: | Phaeodactylum tricornutum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002160 |
| Subject Type: | Other organism |
| Subject Species: | Phaeodactylum tricornutum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 21_exudate-9d_1 | SA196336 | FL023938 | exudate | Type |
| 21_exudate-9d_1 | SA196336 | FL023938 | 9 days | Time Point |
Collection:
| Collection ID: | CO002153 |
| Collection Summary: | Spent medium and cell pellets were collected from axenic Phaeodactylum tricornutum cultures at 5 time points: 0, 3, 6, 9, and 12 days after inoculation. Cultures from four replicate flasks were destructively harvested at each collection time point. |
| Sample Type: | Algae |
Treatment:
| Treatment ID: | TR002172 |
| Treatment Summary: | Treatments were different time points during algal growth: 0, 3, 6, 9, and 12 days after inoculation. |
Sample Preparation:
| Sampleprep ID: | SP002166 |
| Sampleprep Summary: | Cell pellets were disrupted for metabolite extraction by resuspension in sterile water, lyophilization, and grinding by vortexing with a sterilized steel ball. Disrupted pellets were resuspended in 50 mL sterile water, filtered, and frozen in the same manner as spent medium. Spent medium and cell pellet samples were extracted by solid phase extraction using Agilent Bond Elut PPL columns. |
Combined analysis:
| Analysis ID | AN003385 | AN003386 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Agilent InfinityLab Poroshell 120 HILIC-Z (150 x 2.1mm,2.7um) | Agilent InfinityLab Poroshell 120 HILIC-Z (150 x 2.1mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | metabolite quantification (peak height or area as denoted in metaboilite table) | metabolite quantification (peak height or area as denoted in metaboilite table) |
Chromatography:
| Chromatography ID: | CH002502 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (150 x 2.1mm,2.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003152 |
| Analysis ID: | AN003385 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolite identifications w ere obtained using Metabolite Atlas (https://github.com/biorack/metatlas) to identify features with m/z , retention time, and fragmentation spectra that matched to a library of analytical standards run in our lab under the same LC-MS/MS analysis conditions. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003153 |
| Analysis ID: | AN003386 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolite identifications w ere obtained using Metabolite Atlas (https://github.com/biorack/metatlas) to identify features with m/z , retention time, and fragmentation spectra that matched to a library of analytical standards run in our lab under the same LC-MS/MS analysis conditions. |
| Ion Mode: | NEGATIVE |
