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MB Sample ID: SA196476
Local Sample ID: | 1wM_6 |
Subject ID: | SU002163 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002163 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
1wM_6 | SA196476 | FL023969 | 1-week | time point |
Collection:
Collection ID: | CO002156 |
Collection Summary: | Plasma was obtained from the blood samples upon centrifugation and stored at −80 °C until analysis. |
Sample Type: | Blood (plasma) |
Treatment:
Treatment ID: | TR002175 |
Treatment Summary: | The mouse model was induced via MCAO using a 6-0 nylon monolayer suture. Animals were anesthetized with 2% isoflurane in 30% oxygen and 70% nitrous oxide using a face mask. The mice were anesthetized, and blood was collected by retro-orbital bleeding using sterile capillary tubes. |
Sample Preparation:
Sampleprep ID: | SP002169 |
Sampleprep Summary: | plasma samples were processed through a simple protein precipitation technique using 150 µL of ice-cold methanol using plasma methanol ratio of 1:3. After the addition of methanol, the mixture was then vortexed and centrifuged at 20,800×g for 10 min at 4 °C. |
Combined analysis:
Analysis ID | AN003393 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Orbitrap Velos Pro mass spectrometer system |
Column | Waters ACQUITY UPLC BEH C18 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Velos LTQ Orbitrap |
Ion Mode | NEGATIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH002508 |
Instrument Name: | Orbitrap Velos Pro mass spectrometer system |
Column Name: | Waters ACQUITY UPLC BEH C18 |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003160 |
Analysis ID: | AN003393 |
Instrument Name: | Thermo Velos LTQ Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |