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MB Sample ID: SA198757

Local Sample ID:194
Subject ID:SU002171
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Human Inclusion Criteria:hospedalized COVID-19 patients

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Subject:

Subject ID:SU002171
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Human Inclusion Criteria:hospedalized COVID-19 patients

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
194SA198757FL024181ModerateGrade of severity
194SA198757FL024181Post-COVID-75Group

Collection:

Collection ID:CO002164
Collection Summary:The analysed were done on EDTA-plasma. All the samples were collected, processed and stored following the ISO standards (ISO 23118:2021), designed for high quality biological samples for metabolomic analysis
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002183
Treatment Summary:not applicable

Sample Preparation:

Sampleprep ID:SP002177
Sampleprep Summary:Frozen EDTA-plasma samples were thawed at room temperature and shaken before use. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis.

Analysis:

MB Sample ID:SA198757
Analysis ID:AN003405
Laboratory Name:Metabolomics
Analysis Type:NMR
Num Factors:102
Num Metabolites:139
Units:uM

NMR:

NMR ID:NM000231
Analysis ID:AN003405
Instrument Name:Bruker 600MHZ
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
NMR Probe:5 mm PATXI 1H−13C−15N and 2H-decoupling probe
NMR Tube Size:5 mm
Pulse Sequence:noesygp1d
Water Suppression:water peak suppression
Dummy Scans:4
Acquisition Time:2.7 s
Relaxation Delay:4 s
Spectral Width:18,028 Hz
Num Data Points Acquired:98000
Line Broadening:0.3
Baseline Correction Method:automatic baseline correction
Chemical Shift Ref Std:doublet at 5.24 ppm
Binned Increment:0.2 ppm
Binned Data Excluded Range:4.5-5.5 ppm
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