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MB Sample ID: SA198757
| Local Sample ID: | 194 |
| Subject ID: | SU002171 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Human Inclusion Criteria: | hospedalized COVID-19 patients |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002171 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
| Human Inclusion Criteria: | hospedalized COVID-19 patients |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 194 | SA198757 | FL024181 | Moderate | Grade of severity |
| 194 | SA198757 | FL024181 | Post-COVID-75 | Group |
Collection:
| Collection ID: | CO002164 |
| Collection Summary: | The analysed were done on EDTA-plasma. All the samples were collected, processed and stored following the ISO standards (ISO 23118:2021), designed for high quality biological samples for metabolomic analysis |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002183 |
| Treatment Summary: | not applicable |
Sample Preparation:
| Sampleprep ID: | SP002177 |
| Sampleprep Summary: | Frozen EDTA-plasma samples were thawed at room temperature and shaken before use. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis. |
Analysis:
| MB Sample ID: | SA198757 |
| Analysis ID: | AN003405 |
| Laboratory Name: | Metabolomics |
| Analysis Type: | NMR |
| Num Factors: | 102 |
| Num Metabolites: | 139 |
| Units: | uM |
NMR:
| NMR ID: | NM000231 |
| Analysis ID: | AN003405 |
| Instrument Name: | Bruker 600MHZ |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5 mm PATXI 1H−13C−15N and 2H-decoupling probe |
| NMR Tube Size: | 5 mm |
| Pulse Sequence: | noesygp1d |
| Water Suppression: | water peak suppression |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.7 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 18,028 Hz |
| Num Data Points Acquired: | 98000 |
| Line Broadening: | 0.3 |
| Baseline Correction Method: | automatic baseline correction |
| Chemical Shift Ref Std: | doublet at 5.24 ppm |
| Binned Increment: | 0.2 ppm |
| Binned Data Excluded Range: | 4.5-5.5 ppm |
