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MB Sample ID: SA203117
Local Sample ID: | m08b |
Subject ID: | SU002202 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides xylanisolvens |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002202 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides xylanisolvens |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
m08b | SA203117 | FL025019 | 4 | Cage |
m08b | SA203117 | FL025019 | Germfree | Microbiota |
m08b | SA203117 | FL025019 | NA-supplementeded | Diet |
m08b | SA203117 | FL025019 | NA | Age (months) |
m08b | SA203117 | FL025019 | NA | WLZ |
m08b | SA203117 | FL025019 | NA | Health phenotype |
Collection:
Collection ID: | CO002195 |
Collection Summary: | Cecal contents were collected on 10 uL plastic inoculation loops at the time of sacrifice, snap frozen in liquid nitrogen, and stored at -80 °C. Aliquots were kept frozen, removed from the loops and weighed. |
Sample Type: | Cecum |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002214 |
Treatment Summary: | Germ-free C57BL/6J mice were reared in plastic flexible film gnotobiotic isolators (Class Biologically Clean) at 23 °C under a strict 12-hour light cycle (lights on at 0600h, off at 1800h). Animals were weaned onto an autoclaved, low-fat, plant polysaccharide-rich chow (catalog number 2018S, Envigo) that was administered ad libitum. Five days prior to colonization, 8-week-old male mice were switched to the NAD precursor-sufficient diet; 2 days later, they were either placed on a NAD precursor-deficient diet, or a NA-supplemented diet or were continued on the NAD precursor-sufficient diet. |
Sample Preparation:
Sampleprep ID: | SP002208 |
Sampleprep Summary: | 10 uL of ice-cold methanol was added per mg of sample, which was then thawed on ice. The mixture was subsequently shaken for two minutes in a BioSpec bead beater. Samples were then centrifuged for 10 minutes at 3,000 x g at 4 °C; a 150 uL aliquot of the supernatant was transferred to a new tube containing 150 uL ice-cold chloroform, then mixed by pulse vortexing and centrifuged for 10 minutes at 3,000 x g at 4 °C. 100 uL of the methanol fraction was transferred to a new tube containing 100 uL of ice-cold chloroform, mixed by pulse-vortexing, and centrifuged for 10 minutes at 3,000 x g at 4 °C. 70 uL was transferred to a new tube and lyophilized in a Labconco SpeedVac. |
Combined analysis:
Analysis ID | AN003465 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters Atlantis C18 |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | pmol/mg |
Chromatography:
Chromatography ID: | CH002559 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Atlantis C18 |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003226 |
Analysis ID: | AN003465 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Metabolites were quantified by MassHunter quantitative analysis tool (Agilent) |
Ion Mode: | POSITIVE |