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MB Sample ID: SA204088
Local Sample ID: | 23ade_side |
Subject ID: | SU002205 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002205 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
23ade_side | SA204088 | FL025048 | adeside | Tissue type |
Collection:
Collection ID: | CO002198 |
Collection Summary: | The patient tissue specimens from 79 NSCLC patients including the two most prevalent histological subtypes adenocarcinoma (ade) and squamous cell (squ) tumor (65 ade patients with age range 36-84; 14 squ patients with age range 49-78) were obtained from Beijing Hospital. Primary tumor tissues, paired adjacent non-cancerous tissues (ANT) (within 2 cm apart from tumor edge) and distant normal tissues (DNT) ( > 2 cm apart from tumor edge) were surgically resected and frozen at −80 °C until metabolite extraction. The study was approved by the Research Ethics Committee of Beijing Hospital (2019BJYYEC-206-02) and also approved by the Ocean University of China (OUC-HM-2022-002). |
Sample Type: | Lung |
Collection Method: | Surgical resect |
Collection Location: | Beijing Hospital |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002217 |
Treatment Summary: | No. Only cancer and noncancerous tissues. |
Sample Preparation:
Sampleprep ID: | SP002211 |
Sampleprep Summary: | Frozen tissue samples were thawed on ice. Each tissue sample was cut into small pieces and accurately weighed. One mL of precooled 80% methanol was added to 100 mg of tissue and the sample mixture was homogenized (60 Hz, 90 seconds). In a tissue homogenizer with precooled module, followed by vortex-mixing for 1 min and incubation at -80 °C for 4 h to allow for sufficient metabolite extraction. Subsequently, the samples were centrifuged at 14,000 × g for 10 min at 4 °C, and the supernatant was collected and dried under vacuum. Sample blanks were prepared using water following the same experimental procedure. Before analysis, dried metabolites were reconstituted with 100 μL LC-MS grade water, vortex-mixed, and centrifuged at 14,000 × g for 10 min to remove insoluble particles prior to UPLC-HRMS analysis. Quality control (QC) samples were prepared by pooling a small volume from samples with enough volume. |
Combined analysis:
Analysis ID | AN003471 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo LTQ XL |
Ion Mode | POSITIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH002562 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003232 |
Analysis ID: | AN003471 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | capillary temperature 300 °C, capillary voltage 20 V, tube lens 20 V, spray voltage 4.0 kV for positive ion mode, auxiliary gas, sheath gas and sweep gas flow rate 10, 40, and 0 arbitrary units. Progenesis QI version 2.4 was used for raw data processing. FORTRAN based home-built secondary metabolomics data processing was applied for artefact removal, blank correction, QC -based signal correction and normalization, etc. |
Ion Mode: | POSITIVE |